Experimental tests to definitively determine ubiquitylation of a substrate

被引：34
作者：
Bloom, J
Pagano, M
机构：
[1] NYU, Sch Med, Dept Pathol, New York, NY 10012 USA
[2] NYU, Inst Canc, New York, NY USA
来源：
UBIQUITIN AND PROTEIN DEGRADATION, PT B | 2005年 / 399卷
关键词：
D O I：
10.1016/S0076-6879(05)99017-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Ubiquitin-mediated proteolysis is a major pathway of protein degradation that regulates numerous cellular processes. An understanding of the circumstances that contribute to the ubiquitylation of a specific protein can yield vast insight into its regulation. This article examines multiple procedures that explain whether a protein is ubiquitylated and suggests methods to investigate the factors that specifically target the substrate for ubiquitylation, as well as the site of ubiquitin conjugation.
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页码：249 / 266
页数：18
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