JNK signaling pathway is involved in piperlongumine-mediated apoptosis in human colorectal cancer HCT116 cells

被引：29

作者：

Li, Wen ^{[1
,2
,3
,4
,5
]}

Wen, Chuangyu ^{[1
,2
,3
,4
]}

Bai, Haiyan ^{[6
]}

Wang, Xiaoyan ^{[1
,2
,3
,4
]}

Zhang, Xiaoli ^{[1
,2
]}

Huang, Lanlan ^{[1
,2
,3
,4
]}

Yang, Xiangling ^{[1
,2
,3
,4
]}

Iwamoto, Aikichi ^{[7
]}

Liu, Huanliang ^{[1
,2
,3
,4
]}

机构：

[1] Sun Yat Sen Univ, Guangdong Inst Gastroenterol, Guangzhou 510655, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Affiliated Hosp 6, Guangzhou 510655, Guangdong, Peoples R China

[3] Sun Yat Sen Univ, Guangdong Prov Key Lab Colorectal & Pelv Floor Di, Guangzhou 510655, Guangdong, Peoples R China

[4] Sun Yat Sen Univ, Inst Human Virol, Guangzhou 510080, Guangdong, Peoples R China

[5] Guangzhou Med Univ, Guangdong Prov Key Lab Allergy & Immunol, Guangzhou 511436, Guangdong, Peoples R China

[6] Sun Yat Sen Univ, Ctr Canc, State Key Lab Oncol South China, Guangzhou 510080, Guangdong, Peoples R China

[7] Univ Tokyo, Inst Med Sci, Adv Clin Res Ctr, Tokyo 1088639, Japan

来源：

ONCOLOGY LETTERS | 2015年 / 10卷 / 02期

关键词：

piperlongumine; colorectal cancer; c-Jun NH2-terminal kinases; apoptosis; phosphorylation; SP600125; C-JUN; INHIBITS PROLIFERATION; ERK; PHOSPHORYLATION; ACTIVATION; INDUCTION; STRESS; DEATH;

D O I：

10.3892/ol.2015.3371

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Piperlongumine (PPLGM), an alkaloid isolated from the long pepper (Piper longum L.), can selectively trigger cancer cell death in colorectal cancer cells. The present study investigated whether the c-Jun NH2-terminal kinase (JNK) signaling pathway is involved in PPLGM-induced apoptosis in the human colorectal cancer HCT116 cell line. The results demonstrated that PPLGM reduced the cell viability and induced cell apoptosis in a time- and concentration-dependent manner, without a significant effect on cell cycle distribution. Meanwhile, treatment with 10 mu M PPLGM resulted in JNK activation within 1 h, and a marked and sustained increase in c-Jun phosphorylation in the HCT116 cells. In addition, SP600125, a general inhibitor of JNK, inhibited PPIGM-induced apoptosis in the HCTH6 cells by inhibiting PPI,GM-induced c-Jun phosphorylation. Altogether, it can be concluded that the.INK signaling pathway, at least in part, is involved in PPLGM-mediated apoptosis in HCI116 cells.

引用

页码：709 / 715

页数：7

共 32 条

[31] OPPOSING EFFECTS OF ERK AND JNK-P38 MAP KINASES ON APOPTOSIS [J].