Extracellular matrix structure after heart transplantation

被引:2
作者
Schacherer, C [1 ]
Koops, D [1 ]
Wiemer, J [1 ]
Hartmann, A [1 ]
Weis, M [1 ]
Klepzig, H [1 ]
Zeiher, AM [1 ]
Olbrich, HG [1 ]
机构
[1] J W Goethe Univ, Dept Med, Div Cardiol, D-60590 Frankfurt, Germany
关键词
extracellular matric structure; heart transplantation; collagen I;
D O I
10.1016/S0167-5273(98)00341-6
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Following heart transplantation remodeling of the donor heart causes changes in the extracellular myocardial matrix. We investigated 20 right ventricular endomyocardial biopsies taken 17+/-4 days (group I, n=9) and 63+/-13 days (group II, n=11) after heart transplantation from 16 patients transplanted for end-stage cardiomyopathy (15 dilated/1 ischemic). Immunohistochemical staining for collagen I, collagen III, collagen IV, and fibronectin was used. Evaluation was performed ata magnification of 400X using a computer-assisted image analyzing system measuring the relative area stained by the immunoperoxidase method, the number of cells in the given area, and the total area. Collagen I per cell was 13.9+/-5.9 mu m(2) in group I and increased significantly 66+/-13 days after heart transplantation in the perimysium around the myocardial cells as well as in the endocardium to 49.9+/-15.1 mu m(2) (P<0.05). No quantitative change in collagen III was noted (75.7+/-12.4 versus 75.5+/-16.0 mu m(2) n.s.). Collagen IV was found in the perimysial, in the capillary bed and in the vascular network. Significant quantitative change in the amount of collagen IV was not found (64.1+/-12.6 versus 61.0+/-8.9 mu m(2)). Fibronectin was found in the entire perimysial extracellular matrix and in the endocardium in relationship with collagen I and III. An increased amount of fibronectin from 87.09+/-9.9 mu m(2) (group I) to 140.8+/-17.9 mu m(2) (group II, P<0.05) was found. The cell area and cell diameters were not significantly different (group I; cell area 772+/-227 mu m(2), diameter 31.3 mu m; group II; cell area 776+/-224 mu m(2), diameter 31.4 mu m).y It is concluded that remodeling of the donor heart after transplantation is characterized by a specific increase in collagen I and fibronectin, whereas a change in other collagen subtypes was not observed. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:115 / 120
页数:6
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