Inhibition of Cell-Free Translation and Replication of Tobacco Mosaic Virus RNA by Exogenously Added 5′-Proximal Fragments of the Genomic RNA

被引：0

作者：

Yoshida, Tetsuya ^{[1
]}

Ishikawa, Masayuki ^{[1
]}

Ishibashi, Kazuhiro ^{[1
]}

机构：

[1] NARO, Div Plant Mol Regulat Res, Inst Agrobiol Sci, 2-1-2 Kannondai, Tsukuba, Ibaraki 3058602, Japan

来源：

VIRUSES-BASEL | 2022年 / 14卷 / 09期

基金：

日本学术振兴会;

关键词：

tobacco mosaic virus; RNA virus; RNA replication; translation; IDENTIFICATION; PROTEIN; BINDS; GENE; 5'-LEADER; SEQUENCE;

D O I：

10.3390/v14091962

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Replication proteins of tobacco mosaic virus (TMV), a positive-sense RNA virus, cotranslationally bind to a 5'-proximal similar to 70-nucleotide (nt) region of the genomic RNA, referred to as the nuclease-resistant (NR) region for replication template selection. Therefore, disruption of the interaction between the viral replication proteins and viral genomic RNA is expected to inhibit the replication of TMV. In this study, we demonstrate that the addition of small RNA fragments (18-33 nts in length) derived from different regions within the NR region inhibit the binding of TMV replication proteins to viral RNA and TMV RNA replication in a cell-free system. Intriguingly, some of the small RNA fragments also inhibited the translation of mRNA in a sequence-nonspecific manner. These results highlight the pleiotropic roles of the 5'-proximal region of the TMV genome.

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页数：8

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