Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase, NADP+-dependent xylitol dehydrogenase, and xylulokinase

被引：71

作者：

Matsushika, Akinori ^{[1
]}

Watanabe, Seiya ^{[2
,3
,4
]}

Kodaki, Tsutomu ^{[2
,3
]}

Makino, Keisuke ^{[2
,3
,5
]}

Sawayama, Shigeki ^{[1
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, BTRC, Hiroshima 7370197, Japan

[2] Kyoto Univ, Inst Adv Energy, Kyoto 6110011, Japan

[3] Japan Sci & Technol Agcy, CREST, Kyoto 6110011, Japan

[4] New Energy & Ind Technol Dev Org NEDO, Saiwai Ku, Kanagawa 2128554, Japan

[5] Kyoto Univ, Innovat Collabrat Ctr, Sakyo Ku, Kyoto 6068501, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2008年 / 105卷 / 03期

关键词：

lignocellulosic biomass; fermentation; ethanol; xylose; yeast; xylitol dehydrogenase; xylulokinase;

D O I：

10.1263/jbb.105.296

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We constructed a set of recombinant Saccharomyces cerevisiae strains with xylose-fermenting ability. A recombinant S. cerevisiae strain D-XR/ARSdR/XK, in which protein engineered NADP(+)-dependent XDH was expressed, showed 40% increased ethanol production and 23% decrease in xylitol excretion as compared with the reference strain D-XR/XDH/XK expressing the wild-type XDH.

引用

页码：296 / 299

页数：4

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