Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase, NADP+-dependent xylitol dehydrogenase, and xylulokinase

被引：71
|
作者：
Matsushika, Akinori [1 ]
Watanabe, Seiya [2 ,3 ,4 ]
Kodaki, Tsutomu [2 ,3 ]
Makino, Keisuke [2 ,3 ,5 ]
Sawayama, Shigeki [1 ]
机构：
[1] Natl Inst Adv Ind Sci & Technol, BTRC, Hiroshima 7370197, Japan
[2] Kyoto Univ, Inst Adv Energy, Kyoto 6110011, Japan
[3] Japan Sci & Technol Agcy, CREST, Kyoto 6110011, Japan
[4] New Energy & Ind Technol Dev Org NEDO, Saiwai Ku, Kanagawa 2128554, Japan
[5] Kyoto Univ, Innovat Collabrat Ctr, Sakyo Ku, Kyoto 6068501, Japan
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2008年 / 105卷 / 03期
关键词：
lignocellulosic biomass; fermentation; ethanol; xylose; yeast; xylitol dehydrogenase; xylulokinase;
D O I：
10.1263/jbb.105.296
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We constructed a set of recombinant Saccharomyces cerevisiae strains with xylose-fermenting ability. A recombinant S. cerevisiae strain D-XR/ARSdR/XK, in which protein engineered NADP(+)-dependent XDH was expressed, showed 40% increased ethanol production and 23% decrease in xylitol excretion as compared with the reference strain D-XR/XDH/XK expressing the wild-type XDH.
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页码：296 / 299
页数：4
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