Systematic reverse genetic screening of T-DNA tagged genes in rice for functional genomic analyses: MADS-box genes as a test case

被引：80
作者：
Lee, S
Kim, J
Son, JS
Nam, J
Jeong, DH
Lee, K
Jang, S
Yoo, J
Lee, J
Lee, DY
Kang, HG
An, GH [1 ]
机构：
[1] Pohang Univ Sci & Technol, POSTECH, Div Mol & Life Sci, Natl Res Lab Plant Funct Genom, Pohang 790784, South Korea
[2] Penn State Univ, Inst Mol Evolutionary Genet, University Pk, PA 16802 USA
[3] Penn State Univ, Dept Biol, University Pk, PA 16802 USA
来源：
PLANT AND CELL PHYSIOLOGY | 2003年 / 44卷 / 12期
关键词：
insertional mutagenesis; MADS-box genes; pool screening; rice; T-DNA;
D O I：
10.1093/pcp/pcg156
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
We have generated 47 DNA pools and 235 subpools from 21,049 T-DNA insertion lines of rice. DNA pools of 500-1,000 lines were adequate for screening a T-DNA insertion within a 2-kb region. To examine the efficacy of the DNA pools, we selected MADS-box genes, which play an important role in controlling various aspects of plant development. A total of 34 MIKC-type MADS-box genes have now been identified from rice sequence databases. Our PCR screening for T-DNA insertions within 12 MADS-box genes resulted in the identification of five insertions in four different genes. These DNA pools will be valuable when isolating T-DNA insertional mutants in various rice genes. The DNA pool screening service and the mutant seeds are available upon request to genean@postecb.ac.kr.
引用
收藏
页码：1403 / 1411
页数：9
相关论文
共 60 条
[1]   MADS-box gene evolution beyond flowers: expression in pollen, endosperm, guard cells, roots and trichomes [J].
Alvarez-Buylla, ER ;
Liljegren, SJ ;
Pelaz, S ;
Gold, SE ;
Burgeff, C ;
Ditta, GS ;
Vergara-Silva, F ;
Yanofsky, MF .
PLANT JOURNAL, 2000, 24 (04) :457-466
[2]   T-DNA insertion mutagenesis in Arabidopsis: Going back and forth [J].
AzpirozLeehan, R ;
Feldmann, KA .
TRENDS IN GENETICS, 1997, 13 (04) :152-156
[3]   MADS-box gene expression in lateral primordia, meristems and differentiated tissues of Arabidopsis thaliana roots [J].
Burgeff, C ;
Liljegren, SJ ;
Tapia-López, R ;
Yanofsky, MF ;
Alvarez-Buylla, ER .
PLANTA, 2002, 214 (03) :365-372
[4]   Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana [J].
Chuang, CF ;
Meyerowitz, EM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (09) :4985-4990
[5]   EARLY FLOWERING AND REDUCED APICAL DOMINANCE RESULT FROM ECTOPIC EXPRESSION OF A RICE MADS BOX GENE [J].
CHUNG, YY ;
KIM, SR ;
FINKEL, D ;
YANOFSKY, MF ;
AN, GH .
PLANT MOLECULAR BIOLOGY, 1994, 26 (02) :657-665
[6]   CHARACTERIZATION OF 2 RICE MADS BOX GENES HOMOLOGOUS TO GLOBOSA [J].
CHUNG, YY ;
KIM, SR ;
KANG, HG ;
NOH, YS ;
PARK, MC ;
FINKEL, D ;
AN, GH .
PLANT SCIENCE, 1995, 109 (01) :45-56
[7]   Ac as a tool for the functional genomics of rice [J].
Enoki, H ;
Izawa, T ;
Kawahara, M ;
Komatsu, M ;
Koh, S ;
Kyozuka, J ;
Shimamoto, K .
PLANT JOURNAL, 1999, 19 (05) :605-613
[8]   T-DNA INSERTION MUTAGENESIS IN ARABIDOPSIS - MUTATIONAL SPECTRUM [J].
FELDMANN, KA .
PLANT JOURNAL, 1991, 1 (01) :71-82
[9]   Specific expression of the AGL1 MADS-box gene suggests regulatory functions in Arabidopsis gynoecium and ovule development [J].
Flanagan, CA ;
Hu, Y ;
Ma, H .
PLANT JOURNAL, 1996, 10 (02) :343-353
[10]   Large-scale T-DNA mutagenesis in Arabidopsis for functional genomic analysis [J].
Galbiati M. ;
Moreno M.A. ;
Nadzan G. ;
Zourelidou M. ;
Dellaporta S.L. .
Functional & Integrative Genomics, 2000, 1 (1) :25-34
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