A Novel Pathway of Chlorimuron-Ethyl Biodegradation by Chenggangzhangella methanolivorans Strain CHL1 and Its Molecular Mechanisms

被引:2
|
作者
Yu, Zhixiong [1 ,2 ]
Dai, Yumeng [1 ,3 ]
Li, Tingting [4 ]
Gu, Wu [1 ,3 ]
Yang, Yi [1 ]
Li, Xiang [1 ]
Peng, Pai [4 ]
Yang, Lijie [4 ]
Li, Xinyu [1 ]
Wang, Jian [1 ]
Su, Zhencheng [1 ]
Li, Xu [1 ]
Xu, Mingkai [1 ]
Zhang, Huiwen [1 ]
机构
[1] Chinese Acad Sci, Inst Appl Ecol, Key Lab Pollut Ecol & Environm Engn, Shenyang 110016, Peoples R China
[2] Basic Med Coll, Shenyang Med Coll, Shenyang 100034, Peoples R China
[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[4] Shenyang Res Inst Chem Ind, Shenyang 110021, Peoples R China
基金
中国国家自然科学基金;
关键词
microorganism degradation; sulfonylurea herbicide; contaminated environment; transcriptome; gene editing; DEGRADATION PATHWAY; CONTAMINATED SOIL; ESTERASE SULE; SEQUENCE; REVEALS; BINDING;
D O I
10.3390/ijms23179890
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chlorimuron-ethyl is a widely used herbicide in agriculture. However, uncontrolled chlorimuron-ethyl application causes serious environmental problems. Chlorimuron-ethyl can be effectively degraded by microbes, but the underlying molecular mechanisms are not fully understood. In this study, we identified the possible pathways and key genes involved in chlorimuron-ethyl degradation by the Chenggangzhangella methanolivorans strain CHL1, a Methylocystaceae strain with the ability to degrade sulfonylurea herbicides. Using a metabolomics method, eight intermediate degradation products were identified, and three pathways, including a novel pyrimidine-ring-opening pathway, were found to be involved in chlorimuron-ethyl degradation by strain CHL1. Transcriptome sequencing indicated that three genes (atzF, atzD, and cysJ) are involved in chlorimuron-ethyl degradation by strain CHL1. The gene knock-out and complementation techniques allowed for the functions of the three genes to be identified, and the enzymes involved in the different steps of chlorimuron-ethyl degradation pathways were preliminary predicted. The results reveal a previously unreported pathway and the key genes of chlorimuron-ethyl degradation by strain CHL1, which have implications for attempts to enrich the biodegradation mechanism of sulfonylurea herbicides and to construct engineered bacteria in order to remove sulfonylurea herbicide residues from environmental media.
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页数:16
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