Suppressive Effect of Tetrahydrocurcumin on Pseudomonas aeruginosa Lipopolysaccharide-Induced Inflammation by Suppressing JAK/STAT and Nrf2/HO-1 Pathways in Microglial Cells

被引:16
作者
Lin, Hui-Wen [1 ,2 ]
Chen, Tzu-Chun [3 ]
Yeh, Jui-Hsuan [3 ]
Tsou, Shang-Chun [4 ]
Wang, Inga [5 ]
Shen, Ting-Jing [6 ]
Chuang, Chen-Ju [7 ]
Chang, Yuan-Yen [6 ,8 ]
机构
[1] Asia Univ, Dept Optometry, Taichung 41354, Taiwan
[2] China Med Univ, China Med Hosp Univ, Dept Med Res, Taichung, Taiwan
[3] Chung Shan Med Univ, Inst Med, Taichung 40201, Taiwan
[4] Chung Shan Med Univ, Dept Nutr, Taichung 40201, Taiwan
[5] Univ Wisconsin, Rehabil Sci & Technol, Milwaukee, WI USA
[6] Chung Shan Med Univ, Sch Med, Dept Microbiol & Immunol, Taichung 40201, Taiwan
[7] Kaohsiung Municipal United Hosp, Emergency Dept, Kaohsiung 80457, Taiwan
[8] Chung Shan Med Univ Hosp, Clin Lab, Taichung 40201, Taiwan
关键词
OXIDATIVE STRESS; RAW264.7; CELLS; CURCUMIN; ACTIVATION;
D O I
10.1155/2022/4978556
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Brain inflammation, a pathological feature of neurodegenerative disorders, exhibits elevated microglial activity and increased levels of inflammatory factors. The present study was aimed at assessing the anti-inflammatory response of tetrahydrocurcumin (THC), the primary hydrogenated metabolite of curcumin, which was applied to treat Pseudomonas aeruginosa (P.a.) lipopolysaccharide- (LPS-) stimulated BV2 microglial cells. THC reduced P.a. LPS-induced mortality and the production of inflammatory mediators IL-6, TNF-alpha, MIP-2, IP-10, and nitrite. A further investigation revealed that THC decreased these inflammatory cytokines synergistically with JAK/STAT signaling inhibitors. THC also increased Nrf2/HO-1 signaling transduction which inhibits iNOS/COX-2/pNF kappa B cascades. Additionally, the presence of the HO-1 inhibitor Snpp increased the levels of IP-10, IL-6, and nitrite while THC treatment reduced those inflammatory factors in P.a. LPS-stimulated BV2 cells. In summary, we demonstrated that THC exhibits anti-inflammatory activities in P.a. LPS-induced inflammation in brain microglial cells by inhibiting STAT1/3-dependent NF-kappa B activation and inducing Nrf2-mediated HO-1 expression.
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页数:10
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