An improved polyclonal antiserum for detecting Ryegrass mosaic rymovirus

被引：6
作者：
Webster, DE
Beck, DL
Rabenstein, F
Forster, RLS
Guy, PL
机构：
[1] Univ Otago, Dept Bot, Dunedin, New Zealand
[2] DSIR, Mt Albert Res Ctr, Auckland, New Zealand
[3] Fed Ctr Breeding Res Cultivated Plants, Inst Resistance Res & Pathogen Diagnost, Aschersleben, Germany
来源：
ARCHIVES OF VIROLOGY | 2005年 / 150卷 / 09期
关键词：
D O I：
10.1007/s00705-005-0531-z
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Ryegrass mosaic virus (RGMV) is considered the most serious and widespread virus infecting temperate pasture grasses. The use of visible symptoms to diagnose infection is unreliable and ELISA analysis requires antibodies with broad cross-reactivity. Here we describe the production of a polyclonal antiserum (PAb-cp3'Delta) using a bacterially expressed RGMV coat protein fragment. The PAb-cp3'Delta antiserum is specific for RGMV and recognises RGMV strains from each major phylogenetic cluster. PAb-cp3'Delta may be used in ELISAs for fast, accurate and inexpensive detection of RGMV.
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页码：1921 / 1926
页数：6
相关论文
共 19 条
[1]   EFFECT OF RYEGRASS MOSAIC-VIRUS ON YIELD OF PERENNIAL RYEGRASS SWARDS [J].
ABROOK, J ;
HEARD, AJ .
ANNALS OF APPLIED BIOLOGY, 1975, 80 (02) :163-168
[2]   Effect of nitrogen on interactions between ryegrass mosaic virus (RMV) and Lolium species in mixed swards [J].
Bain, RA ;
Raynor, EL ;
Baker, LJ ;
Bagnall, A .
ANNALS OF APPLIED BIOLOGY, 1998, 132 (02) :313-324
[3]   Temporal dynamics of spread of four viruses within mixed species perennial pastures [J].
Coutts, BA ;
Jones, RAC .
ANNALS OF APPLIED BIOLOGY, 2002, 140 (01) :37-52
[4]   Introduced plant viruses and the invasion of a native grass flora [J].
Davis L.T. ;
Guy P.L. .
Biological Invasions, 2001, 3 (1) :89-95
[5]   HOST RANGE, SYMPTOMS AND EFFECTS ON PASTURE PRODUCTION OF A VICTORIAN ISOLATE OF RYEGRASS MOSAIC POTYVIRUS [J].
EAGLING, DR ;
VILLALTA, O ;
SWARD, RJ .
AUSTRALIAN JOURNAL OF AGRICULTURAL RESEARCH, 1992, 43 (05) :1243-1251
[6]   EFFECT OF RYEGRASS MOSAIC-VIRUS ON THE QUALITY OF PERENNIAL RYEGRASS [J].
HOLMES, SJI .
ANNALS OF APPLIED BIOLOGY, 1979, 91 (01) :75-79
[7]  
*ICTVDB, 2005, DESCR PLANT VIR
[8]   Effects of biotic and abiotic stress on the growth of three genotypes of Lolium perenne with and without infection by the fungal endophyte Neotyphodium lolii [J].
Lewis, GC .
ANNALS OF APPLIED BIOLOGY, 2004, 144 (01) :53-63
[9]   Australian isolates of ryegrass mosaic rymovirus and their relationships [J].
Mackenzie, AM ;
Webster, DE ;
Gibbs, MJ ;
Thomas, BJ ;
Gibbs, AJ .
ARCHIVES OF VIROLOGY, 1999, 144 (02) :309-316
[10]  
PLUMB R, 1980, P 3 C VIR DIS GRAM E, P75
12