Novel Pentadecenyl Tetrazole Enhances Susceptibility of Methicillin-Resistant Staphylococcus aureus Biofilms to Gentamicin

被引：14

作者：

Olson, Krista M. ^{[1
]}

Starks, Courtney M. ^{[1
]}

Williams, Russell B. ^{[1
]}

O'Neil-Johnson, Mark ^{[1
]}

Huang, Zhongping ^{[2
]}

Ellis, Michael ^{[2
]}

Reilly, John E. ^{[2
]}

Eldridge, Gary R. ^{[1
]}

机构：

[1] Sequoia Sci Inc, St Louis, MO 63114 USA

[2] AMRI, Albany, NY 12203 USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2011年 / 55卷 / 08期

关键词：

BACILLUS-SUBTILIS; GENETIC-ANALYSIS; INFECTIONS; VIRULENCE; MULTIPLE; AGENTS;

D O I：

10.1128/AAC.00302-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

One method that bacteria employ to reduce their susceptibility to antibiotics is the formation of biofilms. We developed a robust 6-well plate biofilm assay to evaluate early-stage discovery compounds against methicillin-resistant Staphylococcus aureus (MRSA). Tissue culture-treated 6-well plates were selected for this assay because they facilitate the adherence of MRSA and enable accurate determination of the number of CFU in each well. The MRSA biofilms formed in this assay exhibit increased tolerances to clinically used antibiotics. Using this biofilm assay, we identified a novel potentiator of gentamicin against MRSA biofilms. The combination of gentamicin and pentadecenyl tetrazole is superior to clinically used MRSA antibiotics against these MRSA biofilms. This novel combination also exhibits synergistic effects on MRSA planktonic cells. This plant-derived compound reveals promise for its effectiveness and warrants further lead optimization as an antibiotic and aminoglycoside potentiator.

引用

页码：3691 / 3695

页数：5

共 27 条

[1] Mutation of sarA in Staphylococcus aureus limits biofilm formation [J].