Extraction and Identification of Ginsenoside Re and Its Effects and Mechanism of Protecting Acute Renal Ischemia-reperfusion Injury in Rats

This paper studies the extraction and identification of ginsenoside Re from ginseng fruits and investigates the effect and mechanism of ginsenoside Re of protecting acute renal ischemia-reperfusion injury in rats. Having been smashed, the ginseng fruits were ultrasonically extracted twice with 95% ethanol for 30 min each. Having been concentrated, the solution was dissolved with distilled water and separated by two-column chromatography, of which one was packed with macroporous resin D4020, and the other was packed with macroporous resin D941. The raw product was dissolved with methanol and was purified by elution. on a Si gel column, finally ginsenoside Re was obtained. The structure of the ginsenoside Re was analyzed by the thin-layer chromatography and NMR methods, and HPLC was carried for the content determination. The model of acute renal ischemia-reperfusion injury in rats was established after ischemia for I h and reperfusion for 1 h or 24 h, serum SOD(superoxide dismutase), MDA(malondialdehyde) and plasma TXB2(thromboxane B-2) and 6-keto-PGF(1 alpha) were detected. The results show that it has accurately, fast, convenient merits and so on. Ginsenoside Re has a protective effect on acute renal ischemia-reperfusion injury in rats, the mechanism may be related to improving the imbalance of thromboxane A(2)(TXA(2))/prostacyclin(PGI(2)) and inhibiting lipid peroxidation reaction.