Studying protein dynamics in living cells

被引：928

作者：

Lippincott-Schwartz, J ^{[1
]}

Snapp, E ^{[1
]}

Kenworthy, A ^{[1
]}

机构：

[1] NICHD, Cell Biol & Metab Branch, NIH, Bethesda, MD 20892 USA

来源：

NATURE REVIEWS MOLECULAR CELL BIOLOGY | 2001年 / 2卷 / 06期

关键词：

D O I：

10.1038/35073068

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Since the advent of the green fluorescent protein, the subcellular localization, mobility, transport routes and binding interactions of proteins can be studied in living cells. Live cell imaging, in combination with photobleaching, energy transfer or fluorescence correlation spectroscopy are providing unprecedented insights into the movement of proteins and their interactions with cellular components. Remarkably, these powerful techniques are accessible to non-specialists using commercially available microscope systems.

引用

页码：444 / 456

页数：13

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