Optical skin biopsies by clinical CARS and multiphoton fluorescence/SHG tomography

被引：71
作者：
Koenig, K. [1 ,2 ,3 ]
Breunig, H. G. [3 ]
Bueckle, R. [3 ]
Kellner-Hoefer, M. [3 ]
Weinigel, M. [3 ]
Buettner, E. [4 ]
Sterry, W. [5 ]
Lademann, J. [5 ]
机构：
[1] Univ Calif Irvine, Beckman Laser Inst & Med Clin, Irvine, CA 92697 USA
[2] Univ Saarland, Dept Biophoton & Laser Technol, D-66123 Saarbrucken, Germany
[3] JenLab GmbH, D-07745 Jena, Germany
[4] APE GmbH, D-13053 Berlin, Germany
[5] Charite, Dept Dermatol, D-10117 Berlin, Germany
来源：
LASER PHYSICS LETTERS | 2011年 / 8卷 / 06期
关键词：
CARS; multiphoton tomography; two-photon; skin; femtosecond laser; cosmetics; lipids; autofluorescence; RAMAN-SCATTERING MICROSCOPY; IN-VIVO; HIGH-SENSITIVITY; STRATUM-CORNEUM; SPECTROSCOPY; TISSUE; MICROSPECTROSCOPY; VITRO;
D O I：
10.1002/lapl.201110014
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
The ultimate challenge for early diagnostics is label-free high-resolution intratissue imaging without taking physical biopsies. A novel hybrid femtosecond laser tomograph provides in vivo optical biopsies of human skin based on non-linear excitation of autofluorescence and the detection of lipids and water by CARS. Applications include skin cancer detection, biosafety tests of intradermal nanoparticles, and the testing of anti-aging products.
引用
收藏
页码：465 / 468
页数：4
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共 22 条
  • [1] CARS Diagnostics of Molecular Media under Nanoporous Confinement
    Arakcheev, V. G.
    Valeev, A. A.
    Morozov, V. B.
    Olenin, A. N.
    [J]. LASER PHYSICS, 2008, 18 (12) : 1451 - 1458
  • [2] Tunable narrow band filter for CARS microscopy
    Bergner, G.
    Vater, E.
    Akimov, D.
    Schluecker, S.
    Bartelt, H.
    Dietzek, B.
    Popp, J.
    [J]. LASER PHYSICS LETTERS, 2010, 7 (07) : 510 - 516
  • [3] An in vivo randomized study of human skin moisturization by a new confocal Raman fiber-optic microprobe: Assessment of a glycerol-based hydration cream
    Chrit, L.
    Bastien, P.
    Sockalingum, G. D.
    Batisse, D.
    Leroy, F.
    Manfait, M.
    Hadjur, C.
    [J]. SKIN PHARMACOLOGY AND PHYSIOLOGY, 2006, 19 (04) : 207 - 215
  • [4] A hyperspectral fluorescence lifetime probe for skin cancer diagnosis
    De Beule, P. A. A.
    Dunsby, C.
    Galletly, N. P.
    Stamp, G. W.
    Chu, A. C.
    Anand, U.
    Anand, P.
    Benham, C. D.
    Naylor, A.
    French, P. M. W.
    [J]. REVIEW OF SCIENTIFIC INSTRUMENTS, 2007, 78 (12)
  • [5] Laser-Induced Fluorescence and Reflectance Spectroscopy for the Discrimination of Basal Cell Carcinoma from the Surrounding Normal Skin Tissue
    Drakaki, E.
    Kaselouris, E.
    Makropoulou, M.
    Serafetinides, A. A.
    Tsenga, A.
    Stratigos, A. J.
    Katsambas, A. D.
    Antoniou, C.
    [J]. SKIN PHARMACOLOGY AND PHYSIOLOGY, 2009, 22 (03) : 158 - 165
  • [6] Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy
    Evans, CL
    Potma, EO
    Puoris'haag, M
    Côté, D
    Lin, CP
    Xie, XS
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (46) : 16807 - 16812
  • [7] Confocal Raman Microspectroscopy for Evaluating the Stratum Corneum Removal by 3 Standard Methods
    Foerster, M.
    Bolzinger, M. A.
    Rovere, M. R.
    Damour, O.
    Montagnac, G.
    Briancon, S.
    [J]. SKIN PHARMACOLOGY AND PHYSIOLOGY, 2011, 24 (02) : 103 - 112
  • [8] Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy
    Freudiger, Christian W.
    Min, Wei
    Saar, Brian G.
    Lu, Sijia
    Holtom, Gary R.
    He, Chengwei
    Tsai, Jason C.
    Kang, Jing X.
    Xie, X. Sunney
    [J]. SCIENCE, 2008, 322 (5909) : 1857 - 1861
  • [9] In vivo drug screening in human skin using femtosecond laser multiphoton tomography
    Koenig, K.
    Ehlers, A.
    Stracke, F.
    Riemann, I.
    [J]. SKIN PHARMACOLOGY AND PHYSIOLOGY, 2006, 19 (02) : 78 - 88
  • [10] Clinical multiphoton tomography
    Koenig, Karsten
    [J]. JOURNAL OF BIOPHOTONICS, 2008, 1 (01) : 13 - 23
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