Biotransformation of oleic acid into 10-ketostearic acid by recombinant Corynebacterium glutamicum-based biocatalyst

被引：10
|
作者：
Lee, Byeonghun [1 ]
Lee, Saebom [1 ]
Kim, Hyeonsoo [1 ]
Jeong, Kijun [2 ]
Park, Jinbyung [3 ]
Lee, Eunyeol [4 ]
Lee, Jinwon [1 ]
机构：
[1] Sogang Univ, Dept Chem & Biomol Engn, Seoul 121742, South Korea
[2] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Daejeon 305701, South Korea
[3] Ewha Womans Univ, Dept Food Sci & Engn, Seoul 120750, South Korea
[4] Kyung Hee Univ, Dept Chem Engn, Yongin 446701, Gyeonggi Do, South Korea
来源：
BIOTECHNOLOGY LETTERS | 2015年 / 37卷 / 05期
关键词：
Biocatalysts; Biotransformation; Corynebacterium glutamicum; 10-Ketostearic acid; Oleate hydratase; Oleic acid; Secondary alcohol dehydrogenase; WHOLE CELLS; CONVERSION; EXPRESSION;
D O I：
10.1007/s10529-015-1775-7
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
To produce 10-ketostearic acid from oleic acid. Oleic acid was converted to 10-ketostearic acid by a recombinant Corynebacterium glutamicum ATCC 13032 expressing oleate hydratase from Stenotrophomonas maltophilia and a secondary alcohol dehydrogenase from Micrococcus luteus under the control of a synthetic constitutive promoter. Optimal conditions for 10-ketostearic acid production were pH 7.5 and 30 A degrees C with 5 g cells l(-1) and 2.5 g oleic acid l(-1). Under these conditions, the cells produced 1.96 g 10-ketostearic acid l(-1) from oleic acid in 6 h, with a conversion yield of 78 % (w) and a maximum volumetric productivity of 1.67 g l(-1) h(-1). This is the first report of 10-ketostearic acid production using a recombinant C. glutamicum.
引用
收藏
页码：1101 / 1106
页数：6
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