New reagent for protein-DNA contacts feootprinting

被引：1

作者：

Koval, OA ^{[1
]}

Oleinikova, SB ^{[1
]}

Chernolovskaya, EL ^{[1
]}

Litvak, VV ^{[1
]}

Vlassov, VV ^{[1
]}

机构：

[1] Inst Bioorgan Chem, Lab Nucle Acids Biochem, Novosibirsk 630090, Russia

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2003年 / 22卷 / 5-8期

关键词：

protein-DNA footprinting; active oxygen species; DNA cleavage; Cu2+ ions; o-bromobenzoic acid;

D O I：

10.1081/NCN-120023040

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have found, that the reaction of o-bromobenzoic acid with Cu2+ ions can be used as a source of activated oxygen species capable of cleaving DNA. Possibility to apply this reaction for footprinting the nucleosome core in the reconstituted chromatin was demonstrated.

引用

页码：1587 / 1589

页数：3