Expression of bovine leukemia virus ENV glycoprotein in insect cells by recombinant baculovirus

被引：8
|
作者：
Russo, S
Montermini, L
Berkovitz-Siman-Tov, R
Ponti, W
Poli, G
机构：
[1] Fac Med Vet, Inst Microbiol & Immunol, I-20133 Milan, Italy
[2] Hop Notre Dame, Inst Canc, Dept Neurol, Ctr Rech LC Simard, Montreal, PQ H2L 4MI, Canada
[3] Agr Res Org, Volcani Ctr, Dept Post Harvest Sci Fresh Prod, IL-50250 Bet Dagan, Israel
来源：
FEBS LETTERS | 1998年 / 436卷 / 01期
关键词：
baculovirus; biotechnology; bovine leukemia virus; glycosylation; recombinant glycoprotein;
D O I：
10.1016/S0014-5793(98)00951-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The gp51-p30 glycoprotein constituting BLV envelope was expressed in Sf-21 insect cells by means of recombinant baculoviruses. Post-infection cell lysates mere analyzed, in order to define the immunologic reactivity of recombinant products. Oligosaccharide chains, containing N-acetylglucosamine, mannose, galactose and sialic acid mere found on recombinant gp51-p30. In order to investigate the timing of transcription and translation of the glycoprotein, kinetic assays were carried out on cell lysates and directly in situ on Sf-21 cells during the course of baculovirus infection, The use of different solubilizing reagents mas also evaluated in order to rescue recombinant glycoprotein from its subcellular location. (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码：11 / 16
页数：6
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