Modeling of Counter Current Monoclonal Antibody Extraction using Aqueous Two-Phase Systems

被引：0

作者：

Samatou, Joachim Ahmed ^{[1
]}

Wentink, Annebart Engbert ^{[1
]}

Rosa, Paula Alexandra J. ^{[2
]}

Azevedo, Ana Margarida ^{[2
]}

Aires-Barros, Maria Raquel ^{[2
]}

Baecker, Werner ^{[3
]}

Gorak, Andrzej ^{[1
]}

机构：

[1] Univ Dortmund, Emil Figge Str 70, D-44227 Dortmund, Germany

[2] Ctr Biol & Chem Engn, Inst Super Tecn, Lisbon, Portugal

[3] Bayer Technol Serv, Leverkusen, Germany

来源：

17TH EUROPEAN SYMPOSIUM ON COMPUTER AIDED PROCESS ENGINEERING | 2007年 / 24卷

关键词：

Monoclonal antibody; aqueous two-phase; extraction; purification;

D O I：

暂无

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Design of chemical processes is usually based on rigorous modeling of unit operations. Unfortunately, the use of physically grounded models in biotechnological applications is rare since their design is mainly based on heuristics and experiments. In this work a computer aided design method is presented for modeling of aqueous two-phase extraction of monoclonal antibodies (MAbs). A conventional counter current extractor is compared with a fractional extractor in terms of purity and concentration of MAbs in the extract. The purity of MAbs increased from 85% in the conventional to almost 100% in the fractional extractor.

引用

页码：935 / 940

页数：6

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