Development, characterization, and cross-amplification of polymorphic microsatellite markers for North American Trachymyrmex and Mycetomoellerius ants

被引：4

作者：

Matthews, Alix E. ^{[1
]}

Rowan, Chase ^{[1
]}

Stone, Colby ^{[1
]}

Kellner, Katrin ^{[1
]}

Seal, Jon N. ^{[1
]}

机构：

[1] Univ Texas Tyler, Dept Biol, Tyler, TX 75799 USA

来源：

BMC RESEARCH NOTES | 2020年 / 13卷 / 01期

基金：

美国国家科学基金会;

关键词：

Attini; Fungus-gardening ant; Intraspecific genetic diversity; Microsatellites; Population genetics; POPULATION GENETIC-STRUCTURE; MALE-BIASED DISPERSAL; FUNGUS-GARDENING ANT; LEAFCUTTER ANT; SOFTWARE; PHYLOGEOGRAPHY; FORMICIDAE; SNP; IDENTIFICATION; SEQUENCES;

D O I：

10.1186/s13104-020-05015-3

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

ObjectiveThe objective of this study is to develop and identify polymorphic microsatellite markers for fungus-gardening (attine) ants in the genus Trachymyrmex sensu lato. These ants are important ecosystem engineers and have been a model group for understanding complex symbiotic systems, but very little is understood about the intraspecific genetic patterns across most North American attine species. These microsatellite markers will help to better study intraspecific population genetic structure, gene flow, mating habits, and phylogeographic patterns in these species and potentially other congeners.ResultsUsing next-generation sequencing techniques, we identified 17 and 12 polymorphic microsatellite markers from T. septentrionalis and Mycetomoellerius (formerly Trachymyrmex) turrifex, respectively, and assessed the genetic diversity of each marker. We also analyzed the cross-amplification success of the T. septentrionalis markers in two other closely related Trachymyrmex species, and identified 10 and 12 polymorphic markers for T. arizonensis and T. pomonae, respectively.

引用

页数：7

共 56 条

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