Site-directed circular dichroism of proteins:: 1Lb bands of Trp resolve position-specific features in tear lipocalin

被引:11
作者
Gasymov, Oktay K.
Abduragimov, Adil R.
Glasgow, Ben J. [1 ]
机构
[1] Univ Calif Los Angeles, Sch Med, Dept Pathol, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Sch Med, Dept Ophthalmol, Los Angeles, CA 90095 USA
关键词
tear lipocalin; Lipocalin-1; von Ebner's gland protein Trp; vibronic band; circular dichroism; near-UV CD; site-directed circular dichroism;
D O I
10.1016/j.ab.2007.11.002
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The absorption spectra of N-acetyl-L-tryptophanamide in various solvents were resolved into the sums of the L-1(a) and L-1(b) components. The relative intensities of the 0-0 transitions of the L-1(b) bands correlate linearly with the solvent polarity values (E-T(N)). A novel strategy that uses a set of the experimental L-1(b) bands was employed to resolve the near-UV circular dichroism (CD) spectra of tryptophanyl residues. Resolved spectral parameters from the single-tryptophan mutants of tear lipocalin (TL), F99W and Y87W, corroborate the fluorescence and structural data of TL. Analysis of the L-1(b) bands of the Trp CD spectra in proteins is a valuable tool to obtain the local features. The dimethyl sulfoxide (DMSO)-like L-1(b) band of Trp CD spectra may be used as a "fingerprint" to identify the tryptophanyl side chains in situations where the benzene rings of Trp have van der Waals interactions with the side chains of its nearest neighbor. In addition, the signs and intensities of the components hold information about the side chain conformations and dynamics in proteins. Combined with Trp mutagenesis, this method, which we call site-directed circular dichroism, is broadly applicable to various proteins to obtain the position-specific data. (C) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码:386 / 395
页数:10
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