Structure, function, and stability of enzymes covalently attached to single-walled carbon nanotubes

被引：145

作者：

Asuri, Prashanth ^{[1
]}

Bale, Shyam Sundhar ^{[1
]}

Pangule, Ravindra C. ^{[1
]}

Shah, Dhiral A. ^{[1
]}

Kane, Ravi S. ^{[1
]}

Dordick, Jonathan S. ^{[1
]}

机构：

[1] Rensselaer Polytech Inst, Rensselaer Nanotechnol Ctr, Ctr Biotechnol & Interdisciplinary Studies, Dept Biol & Chem Engn, Troy, NY 12180 USA

来源：

LANGMUIR | 2007年 / 23卷 / 24期

关键词：

D O I：

10.1021/la702091c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We describe the structure, activity, and stability of enzymes covalently attached to single-walled carbon nanotubes (SWNTs). Conjugates of SWNTs with three functionally unrelated enzymes-horseradish peroxidase, subtilisin Carlsberg, and chicken egg white lysozyme-were found to be soluble in aqueous solutions. Furthermore, characterization of the secondary and tertiary structure of the immobilized proteins by circular dichroism and fluorescence spectroscopies, respectively, and determination of enzyme kinetics revealed that the enzymes retained a high fraction of their native structure and activity upon attachment to SWNTs. The SWNT-enzyme conjugates were also more stable in guanidine hydrochloride (GdnHCl) and at elevated temperatures relative to their solution counterparts. Thus, these protein conjugates represent novel preparations that possess the attributes of both soluble enzymes-high activity and low diffusional resistance-and immobilized enzymes-high stability-making them attractive choices' for applications ranging from diagnostics and sensing to drug delivery.

引用

页码：12318 / 12321

页数：4

共 23 条

[1] Directed assembly of carbon nanotubes at liquid-liquid interfaces: Nanoscale conveyors for interfacial biocatalysis [J].

Asuri, P ;

Karajanagi, SS ;

Dordick, JS ;

Kane, RS .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (04) :1046-1047

[2] Increasing protein stability through control of the nanoscale environment [J].

Asuri, Prashanth ;

Karajanagi, Sandeep S. ;

Yang, Hoichang ;

Yim, Tae-Jin ;

Kane, Ravi S. ;

Dordick, Jonathan S. .

LANGMUIR, 2006, 22 (13) :5833-5836

[3] Polymer-nanotube-enzyme composites as active antifouling films [J].

Asuri, Prashanth ;

Karajanagi, Sandeep S. ;

Kane, Ravi S. ;

Dordick, Jonathan S. .

SMALL, 2007, 3 (01) :50-53

[4] Water-soluble carbon nanotube-enzyme conjugates as functional biocatalytic formulations [J].

Asuri, Prashanth ;

Karajanagi, Sandeep S. ;

Sellitto, Edward ;

Kim, Dae-Yun ;

Kane, Ravi S. ;

Dordick, Jonathan S. .

BIOTECHNOLOGY AND BIOENGINEERING, 2006, 95 (05) :804-811

[5] Near-infrared optical sensors based on single-walled carbon nanotubes [J].

Barone, PW ;

Baik, S ;

Heller, DA ;

Strano, MS .

NATURE MATERIALS, 2005, 4 (01) :86-U16

[6] Enzyme-coated carbon nanotubes as single-molecule biosensors [J].

Besteman, K ;

Lee, JO ;

Wiertz, FGM ;

Heering, HA ;

Dekker, C .

NANO LETTERS, 2003, 3 (06) :727-730

[7] Noncovalent functionalization of carbon nanotubes for highly specific electronic biosensors [J].

Chen, RJ ;

Bangsaruntip, S ;

Drouvalakis, KA ;

Kam, NWS ;

Shim, M ;

Li, YM ;

Kim, W ;

Utz, PJ ;

Dai, HJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (09) :4984-4989

[8] Binding of an anti-fullerene IgG monoclonal antibody to single wall carbon nanotubes [J].

Erlanger, BF ;

Chen, BX ;

Zhu, M ;

Brus, L .

NANO LETTERS, 2001, 1 (09) :465-467

[9] Achieving individual-nanotube dispersion at high loading in single-walled carbon nanotube composites [J].

Graff, RA ;

Swanson, JP ;

Barone, PW ;

Baik, S ;

Heller, DA ;

Strano, MS .

ADVANCED MATERIALS, 2005, 17 (08) :980-+

[10] Nanotube molecular transporters: Internalization of carbon nanotube-protein conjugates into mammalian cells [J].

Kam, NWS ;

Jessop, TC ;

Wender, PA ;

Dai, HJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (22) :6850-6851

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