The roles of steroidogenic factor-1 in reproductive function

The cytochrome P450 steroid hydroxylases are expressed in a tissue-specific and developmentally regulated manner, and the orphan nuclear receptor steroidogenic factor 1 (SF-1) participates in both aspects of regulated expression. SF-1 is expressed in mouse embryos from the inception of adrenal and gonadal development, suggesting that SF-1 plays important roles in their differentiation. SF-1 is also expressed in the embryonic pituitary gland and ventral diencephalon, suggesting additional roles within the hypothalamic-pituitary-steroidogenic organ axis. To examine the roles of SF-1 in vivo, we used targeted gene disruption to ''knock out'' the mouse gene encoding SF-1. Analyses of these knockout mice established roles of SF-1 at levels of endocrine development that include adrenal and gonadal differentiation, pituitary gonadotrope function, and formation of the ventromedial hypothalamic nucleus. These results indicate that SF-1 plays multiple roles in endocrine development that are essential for reproduction.