An improved protocol for pulsed-field gel electrophoresis typing of Clostridium difficile

被引：36

作者：

Alonso, R ^{[1
]}

Martín, A ^{[1
]}

Peláez, T ^{[1
]}

Marín, M ^{[1
]}

Rodríguez-Creixéms, M ^{[1
]}

Bouza, E ^{[1
]}

机构：

[1] Hosp Gen Univ Gregorio Maranon, Dept Clin Microbiol & Infect Dis, Madrid 28007, Spain

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 2005年 / 54卷 / 02期

关键词：

STRAINS; PCR; MULTICENTER; HOSPITALS;

D O I：

10.1099/jmm.0.45808-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Pulsed-field gel electrophoresis (PFGE) is the 'gold standard' technique for bacterial typing and has proved to be discriminatory and reproducible for typing Clostridium difficile. Nevertheless, a high proportion of strains are non-typable by this technique due to the degradation of the DNA during the process. The introduction of several modifications in the PFGE standard procedure increased typability from 40% (90 isolates) to 100% (220 isolates) while maintaining the high degree of discrimination and reproducibility of the technique.

引用

页码：155 / 157

页数：3

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