Detailed analysis of the D-galactose catabolic pathways in Aspergillus niger reveals complexity at both metabolic and regulatory level

被引：13

作者：

Chroumpi, Tania ^{[1
,2
]}

Martinez-Reyes, Natalia ^{[1
,2
]}

Kun, Roland S. ^{[1
,2
]}

Peng, Mao ^{[1
,2
]}

Lipzen, Anna ^{[3
]}

Ng, Vivian ^{[3
]}

Tejomurthula, Sravanthi ^{[3
]}

Zhang, Yu ^{[3
]}

Grigoriev, Igor, V ^{[3
,4
]}

Makela, Miia R. ^{[5
]}

de Vries, Ronald P. ^{[1
,2
]}

Garrigues, Sandra ^{[1
,2
,6
]}

机构：

[1] Univ Utrecht, Westerdijk Fungal Biodivers Inst, Fungal Physiol, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands

[2] Univ Utrecht, Fungal Mol Physiol, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands

[3] Lawrence Berkeley Natl Lab, US DOE Joint Genome Inst, Berkeley, CA 94720 USA

[4] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA

[5] Univ Helsinki, Dept Microbiol, POB 56,Viikinkaari 9, Helsinki, Finland

[6] Inst Agroquim & Tecnol Alimentos, Dept Food Biotechnol, Consejo Super Invest Cient, Valencia, Spain

来源：

FUNGAL GENETICS AND BIOLOGY | 2022年 / 159卷

基金：

芬兰科学院;

关键词：

D-galactose catabolism; Leloir pathway; Oxido-reductive D-galactose catabolic pathway; Pentose Catabolic Pathway (PCP); Transcription factors; Aspergillus niger; TRANSCRIPTIONAL ACTIVATOR XLNR; D-XYLOSE REDUCTASE; BETA-GALACTOSIDASE; OXIDOREDUCTIVE PATHWAY; CELLULASE INDUCTION; ALPHA-GALACTOSIDASE; HYPOCREA-JECORINA; GENE; EXPRESSION; DEGRADATION;

D O I：

10.1016/j.fgb.2022.103670

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The current impetus towards a sustainable bio-based economy has accelerated research to better understand the mechanisms through which filamentous fungi convert plant biomass, a valuable feedstock for biotechnological applications. Several transcription factors have been reported to control the polysaccharide degradation and metabolism of the resulting sugars in fungi. However, little is known about their individual contributions, interactions and crosstalk. D-galactose is a hexose sugar present mainly in hemicellulose and pectin in plant biomass. Here, we study D-galactose conversion by Aspergillus niger and describe the involvement of the arabinanolytic and xylanolytic activators AraR and XlnR, in addition to the D-galactose-responsive regulator GalX. Our results deepen the understanding of the complexity of the filamentous fungal regulatory network for plant biomass degradation and sugar catabolism, and facilitate the generation of more efficient plant biomass degrading strains for biotechnological applications.

引用

页数：10

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