Counting of Escherichia coli by a microflow cytometer based on a photonic-microfluidic integrated device

被引：15

作者：

Guo, Tianyi ^{[1
,2
]}

Wei, Yin ^{[3
]}

Xu, Changqing ^{[3
]}

Watts, Benjamin R. ^{[3
]}

Zhang, Zhiyi ^{[4
]}

Fang, Qiyin ^{[1
,3
]}

Zhang, Haiying ^{[2
]}

Selvaganapathy, P. Ravi ^{[5
]}

Deen, M. Jamal ^{[1
,6
]}

机构：

[1] McMaster Univ, Sch Biomed Engn, Hamilton, ON L8S 4L8, Canada

[2] Chinese Acad Sci, Inst Microelect, Beijing, Peoples R China

[3] McMaster Univ, Dept Engn, Hamilton, ON L8S 4L8, Canada

[4] Natl Res Council Canada, Ottawa, ON, Canada

[5] McMaster Univ, Dept Mech Engn, Hamilton, ON L8S 4L8, Canada

[6] McMaster Univ, Dept Elect & Comp Engn, Hamilton, ON L8S 4L8, Canada

来源：

ELECTROPHORESIS | 2015年 / 36卷 / 02期

基金：

加拿大创新基金会; 加拿大自然科学与工程研究理事会;

关键词：

Escherichia coli; Integrated optics; Microflow cytometer; Scattering detection; FLOW-CYTOMETRY; ON-CHIP; RAPID DETECTION; DRINKING-WATER; FLUORESCENCE DETECTION; OPTOFLUIDIC DEVICE; OPTICAL-DETECTION; TOTAL BACTERIA; GROUND-BEEF; APPLE JUICE;

D O I：

10.1002/elps.201400211

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Counting of Escherichia coli DH5 alpha-cell suspensions in PBS is performed using a microflow cytometer based on a photonic-microfluidic integrated device. Side-scattered light signals are used to count the E. coli cells. A detection efficiency of 92% is achieved when compared with the expected count from a hemocytometer. The detection efficiency is correlated to the ratio of sample to sheath flow rates. It is demonstrated that E. coli can be easily distinguished from beads of similar sizes (2-4 mu m) as their scattering intensities are different.

引用

页码：298 / 304

页数：7

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