Development of a Multi-Epitope Vaccine for Mycoplasma hyopneumoniae and Evaluation of Its Immune Responses in Mice and Piglets

被引:11
作者
Li, Gaojian [1 ]
Shu, Jinqi [1 ]
Jin, Jing [1 ]
Shu, Jianhong [1 ,2 ]
Feng, Huapeng [1 ]
Chen, Jian [1 ]
He, Yulong [1 ,2 ]
机构
[1] Zhejiang Sci Tech Univ, Dept Biopharm, Coll Life Sci & Med, Hangzhou 310018, Peoples R China
[2] Shaoxing Biomed Res Inst, Zhejiang Sci Tech Univ, Shaoxing 312000, Peoples R China
关键词
Mycoplasma hyopneumoniae; reverse vaccinology; multi-epitope vaccine design; immunization of mice and piglets; immune response evaluation; B-CELL EPITOPES; PROTEIN-STRUCTURE; PREDICTION; SURFACE; ADHESIN; SERVER; IMMUNIZATION; LIPOPROTEIN; ADJUVANTS; ANTIGENS;
D O I
10.3390/ijms23147899
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mycoplasma hyopneumoniae (Mhp), the primary pathogen causing Mycoplasma pneumonia of swine (MPS), brings massive economic losses worldwide. Genomic variability and post-translational protein modification can enhance the immune evasion of Mhp, which makes MPS prone to recurrent outbreaks on farms, even with vaccination or other treatments. The reverse vaccinology pipeline has been developed as an attractive potential method for vaccine development due to its high efficiency and applicability. In this study, a multi-epitope vaccine for Mhp was developed, and its immune responses were evaluated in mice and piglets. Genomic core proteins of Mhp were retrieved through pan-genome analysis, and four immunodominant antigens were screened by host homologous protein removal, membrane protein screening, and virulence factor identification. One immunodominant antigen, AAV27984.1 (membrane nuclease), was expressed by E. coli and named rMhp597. For epitope prioritization, 35 B-cell-derived epitopes were identified from the four immunodominant antigens, and 10 MHC-I and 6 MHC-II binding epitopes were further identified. The MHC-I/II binding epitopes were merged and combined to produce recombinant proteins MhpMEV and MhpMEVC6His, which were used for animal immunization and structural analysis, respectively. Immunization of mice and piglets demonstrated that MhpMEV could induce humoral and cellular immune responses. The mouse serum antibodies could detect all 11 synthetic epitopes, and the piglet antiserum suppressed the nuclease activity of rMhp597. Moreover, piglet serum antibodies could also detect cultured Mhp strain 168. In summary, this study provides immunoassay results for a multi-epitope vaccine derived from the reverse vaccinology pipeline, and offers an alternative vaccine for MPS.
引用
收藏
页数:22
相关论文
共 50 条
  • [1] Development and evaluation of a multi-epitope subunit vaccine against group B Streptococcus infection
    Zhang, Yumin
    Liang, Song
    Zhang, Shiyu
    Zhang, Shidan
    Yu, Yong
    Yao, Huochun
    Liu, Yongjie
    Zhang, Wei
    Liu, Guangjin
    EMERGING MICROBES & INFECTIONS, 2022, 11 (01) : 2371 - 2382
  • [2] Immune Responses to the Attenuated Mycoplasma hyopneumoniae 168 Strain Vaccine by Intrapulmonic Immunization in Piglets
    Feng Zhi-xin
    Shao Guo-qing
    Liu Mao-jun
    Wu Xu-su
    Zhou Yong-qi
    Gan Yuan
    AGRICULTURAL SCIENCES IN CHINA, 2010, 9 (03): : 423 - 431
  • [3] The immune responses elicited by six recombinant antigens of Mycoplasma hyopneumoniae in mice
    Li, Shiyang
    Yin, Ruiru
    Xiong, Qiyan
    Liu, Maojun
    Wang, Jia
    Zhang, Zhenzhen
    Shao, Guoqing
    Deng, Zhibang
    Feng, Zhixin
    Yu, Yanfei
    VETERINARY MICROBIOLOGY, 2024, 299
  • [4] A candidate multi-epitope vaccine against porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae induces robust humoral and cellular response in mice
    Gao, Zhisong
    Chen, Lingxiao
    Song, Tao
    Pan, Xuan
    Li, Xiaojing
    Lu, Guangzong
    Tang, Yajie
    Wu, Xiang
    Zhao, Baohua
    Zhang, Ran
    VACCINE, 2022, 40 (16) : 2370 - 2378
  • [5] Development of a novel multi-epitope vaccine for brucellosis prevention
    Shang, Kaiyu
    Zhu, Yuejie
    Tian, Tingting
    Shi, Huidong
    Yin, Zhengwei
    He, Yueyue
    Shi, Juan
    Ding, Jianbing
    Zhang, Fengbo
    HELIYON, 2024, 10 (14)
  • [6] A new multi-epitope peptide vaccine induces immune responses and protection against Leishmania infantum in BALB/c mice
    Vakili, Bahareh
    Nezafat, Navid
    Zare, Bijan
    Erfani, Nasrollah
    Akbari, Maryam
    Ghasemi, Younes
    Rahbar, Mohammad Reza
    Hatam, Gholam Reza
    MEDICAL MICROBIOLOGY AND IMMUNOLOGY, 2020, 209 (01) : 69 - 79
  • [7] Design, evaluation, and immune simulation of potentially universal multi-epitope mpox vaccine candidate: focus on DNA vaccine
    Rcheulishvili, Nino
    Mao, Jiawei
    Papukashvili, Dimitri
    Feng, Shunping
    Liu, Cong
    Wang, Xingyun
    He, Yunjiao
    Wang, Peng George
    FRONTIERS IN MICROBIOLOGY, 2023, 14
  • [8] Development of multi-epitope chimeric vaccine against Taenia solium by exploring its proteome: an in silico approach
    Kaur, Rimanpreet
    Arora, Naina
    Jamakhani, Majeed Abdulwahid
    Malik, Shelvia
    Kumar, Pramod
    Anjum, Farhan
    Tripathi, Shweta
    Mishra, Amit
    Prasad, Amit
    EXPERT REVIEW OF VACCINES, 2020, 19 (01) : 105 - 114
  • [9] Immunoinformatics-Aided Design and Evaluation of a Potential Multi-Epitope Vaccine against Klebsiella Pneumoniae
    Dar, Hamza Arshad
    Zaheer, Tahreem
    Shehroz, Muhammad
    Ullah, Nimat
    Naz, Kanwal
    Muhammad, Syed Aun
    Zhang, Tianyu
    Ali, Amjad
    VACCINES, 2019, 7 (03)
  • [10] Evaluation of multi-epitope recombinant protein as a candidate for a contraceptive vaccine
    Mortazavi, Behnam
    Fard, Najaf Allahyari
    Karkhane, Ali Asghar
    Shokrpoor, Sara
    Heidari, Farid
    JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2021, 145