Expression of Nanog gene promotes NIH3T3 cell proliferation

被引：71
|
作者：
Zhang, JY
Wang, X
Chen, B
Suo, GL
Zhao, YH
Duan, ZY
Dai, JW [1 ]
机构：
[1] Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol Dev Biol, Beijing 100080, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 338卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Nanog; cell cycle; cell proliferation; NIH3T3;
D O I：
10.1016/j.bbrc.2005.10.071
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cells are the functional elements in tissue engineering and regenerative medicine. A large number of cells are usually needed for these purposes. However, there are numbers of limitations for in vitro cell proliferation. Nanog is an important self-renewal determinant in embryonic stem cells. However, it remains unknown whether Nanog will influence the cell cycle and cell proliferation of mature cells. In this study, we expressed Nanog in NIH3T3 cells and showed that expression of Nanog in NIH3T3 promoted cells to enter into S phase and enhanced cell proliferation. This suggests that Nanog gene might function in a similar fashion in mature cells as in ES cells. In addition, it may provide an approach for in vitro cell expansion. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码：1098 / 1102
页数：5
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