Identification, biochemical characterization and biological role of defense proteins from common bean genotypes seeds in response to Callosobruchus maculatus infestation

被引：6

作者：

Viega de Andrade, Elisangela Knoblauch ^{[1
]}

Rodrigues, Rosana ^{[1
]}

Vieira Bard, Gabriela da Costa ^{[2
]}

Pereira, Lidia Silva ^{[2
]}

Ventury Baptista, Kayan Eudorico ^{[3
]}

Menezes Cavalcanti, Thamara Figueiredo ^{[1
]}

Amancio Oliveira, Antonia Elenir ^{[3
]}

Melo Souza, Thayna Amanda ^{[2
]}

Gomes, Valdirene Moreira ^{[2
]}

机构：

[1] Univ Estadual Norte Fluminense, Ctr Ciencias & Tecnol Agr, Lab Melhoramento Genet Vegetal, Campos Dos Goytacazes, RJ, Brazil

[2] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Fisiol & Bioquim Microrganismos, Campos Dos Goytacazes, RJ, Brazil

[3] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Quim & Funcao Prot & Peptideos, Campos Dos Goytacazes, RJ, Brazil

来源：

JOURNAL OF STORED PRODUCTS RESEARCH | 2020年 / 87卷

关键词：

Phaseolus vulgaris; Plant defensin; Defense proteins; Seed coat; Proteinase inhibitor; ALPHA-AMYLASE INHIBITORS; STORAGE PROTEINS; PLANT DEFENSINS; TRYPSIN-INHIBITOR; COAT; RESISTANCE; INSECT; PEPTIDES; TOXICITY; FABACEAE;

D O I：

10.1016/j.jspr.2020.101580

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Common bean is a legume of significant socioeconomic importance and is cultivated worldwide. This crop is affected by several pests and diseases, which cause considerable economic losses and reduce yield. In recent years, several studies have demonstrated the role of proteins and peptides with activity against a wide range of insects and pathogens. The objective of this work was to identify defense proteins, such as antimicrobial peptides, protease and amylases inhibitors in common bean genotypes and evaluate the relationship of these proteins with Phaseolus vulgaris seed resistance to Callosobruchus maculatus infestation. Nineteen common bean genotypes were subjected to protein extraction, pH 5.4, and precipitation with ammonium sulfate at 70% saturation. The obtained extracts were separated by tricine gel electrophoresis. Experiments were carried out with natural seeds of common beans and artificial seeds (Vigna unguiculata seeds covered with seed coats of common beans) to evaluate the rate of oviposition and development of the insect species Callosobruchus maculatus. Lipid-transfer proteins were identified in nine genotypes whereas defensins were present in all genotypes. The inhibitory activity of alpha-amylases and trypsin and fungal development were determined in crude extracts (50 mu g mL(-1)). The results also indicated that the extracts from all bean genotypes inhibited the activity of human salivary aamylase and C. maculatus larval a-amylase. Except for the extracts of four genotypes, all other extracts inhibited trypsin activity. None of the extracts from the evaluated bean genotypes inhibited the growth of tested fungi. Natural seeds from all genotypes did not inhibit insect oviposition, however, the larvae did not survive after feeding on these seeds. Artificial seeds containing seed coat flour of all genotypes inhibited the oviposition of C. maculatus, indicating that the seed coat was also repellent to insect. (C) 2020 Elsevier Ltd. All rights reserved.

引用

页数：9