Influence of culture conditions on thermostable lipase production by a thermophilic alkalitolerant strain of Bacillus sp

被引：9

作者：

Kambourova, M ^{[1
]}

Emanuilova, E ^{[1
]}

Dimitrov, P ^{[1
]}

机构：

[1] INST MICROBIOL, SOFIA 1113, BULGARIA

来源：

FOLIA MICROBIOLOGICA | 1996年 / 41卷 / 02期

关键词：

D O I：

10.1007/BF02814690

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of different culture conditions on thermostable lipase production by Bacillus sp. was studied in shake flasks. A maximum enzyme activity of 67-75 nkat/mL was observed in a medium consisting of 0.5 % soybean flour and 0.1 % stearyl glycerol esters or natural fats. A lipase activity of about 117 nkat/ml was established when the cultivation was carried out in a laboratory fermentor at 20 % minimal dissolved oxygen level, the enzyme production being increased 1.5 fold compared to that in a flask culture.

引用

页码：146 / 148

页数：3

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