In vivo imaging of ram spermatozoa in the ewe genital tract using fibered confocal microscopy

被引：3
|
作者：
Druart, X. [1 ]
Cognie, J. [1 ]
Baril, G. [1 ]
Clement, F. [2 ]
Dacheux, J. -L. [1 ]
Gatti, J. -L. [1 ]
机构：
[1] Univ Tours Hares Nationaux, CNRS, UMR 6175, INRA, F-37380 Nouzilly, France
[2] INRIA Paris Rocquencourt, F-78153 Le Chesnay, France
来源：
GYNECOLOGIE OBSTETRIQUE & FERTILITE | 2011年 / 39卷 / 11期
关键词：
Sperm; In vivo imaging; Female genital tract; Transit; Fertility; STORAGE; OVIDUCT;
D O I：
10.1016/j.gyobfe.2011.09.007
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
Sperm transit in the female tract is one of the key factors in the success of fertilization after artificial insemination in sheep species. However, its study is limited by the absence of in vivo imaging methods. The imaging of ram sperm in the female genital tract was made possible using the confocal fibered microscopy and fluorescent stains adapted to spermatozoa. Our results show the active role of the uterotubal junction in the selection of sperm during their transit. (C) 2011 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码：633 / 635
页数：3
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