Whole genome sequencing for drug resistance determination in Mycobacterium tuberculosis

被引：5
|
作者：
Omar, Shaheed, V [1 ]
Joseph, Lavania [1 ]
Said, Halima M. [1 ,2 ]
Ismail, Farzana [1 ,3 ]
Ismail, Nabila [1 ]
Gwala, Thabisile L. [1 ]
Ismail, Nazir A. [1 ,3 ]
机构：
[1] Natl Hlth Lab Serv, Natl Inst Communicable Dis, World Hlth Org TB Supranatl Reference Lab Network, Ctr TB, Johannesburg, South Africa
[2] Univ Free State, Dept Med Microbiol, Bloemfontein, South Africa
[3] Univ Pretoria, Dept Med Microbiol, Pretoria, South Africa
来源：
AFRICAN JOURNAL OF LABORATORY MEDICINE | 2019年 / 8卷 / 01期
关键词：
RIFAMPICIN RESISTANCE; SUSCEPTIBILITY; MUTATIONS; COMPLEX; SYSTEM;
D O I：
10.4102/ajlm.v8i1.801
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
South Africa remains challenged with a high tuberculosis burden accompanied by an increase in drug resistant cases. We assessed the use of the Illumina MiSeq, a next-generation sequencing platform for whole genome sequencing, followed by bioinformatic analysis using a commercial software package to determine resistance to selected drugs used for Mycobacterium tuberculosis treatment in our setting. Whole genome sequencing shows potential as a diagnostic platform for the detection of drug resistance in Mycobacterium tuberculosis with the provision of information for several drugs simultaneously.
引用
收藏
页数：5
相关论文
共 50 条
  • [21] Whole genome enrichment approach for rapid detection of Mycobacterium tuberculosis and drug resistance-associated mutations from direct sputum sequencing
    Soundararajan, Lakshmi
    Kambli, Priti
    Priyadarshini, Sushri
    Let, Biswajit
    Murugan, Sakthivel
    Iravatham, Chitra
    Tornheim, Jeffrey A.
    Rodrigues, Camilla
    Gupta, Ravi
    Ramprasad, V. L.
    TUBERCULOSIS, 2020, 121
  • [22] Evaluation of whole genome sequencing and software tools for drug susceptibility testing of Mycobacterium tuberculosis
    van Beek, J.
    Haanpera, M.
    Smit, P. W.
    Mentula, S.
    Soini, H.
    CLINICAL MICROBIOLOGY AND INFECTION, 2019, 25 (01) : 82 - 86
  • [23] Whole genome sequencing of drug-resistant Mycobacterium tuberculosis isolates in Victoria, Australia
    Dorji, Thinley
    Horan, Kristy
    Sherry, Norelle L.
    Tay, Ee Laine
    Globan, Maria
    Viberg, Linda
    Bond, Katherine
    Denholm, Justin T.
    Howden, Benjamin P.
    Andersson, Patiyan
    INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2024, 138 : 46 - 53
  • [24] Whole genome analysis of extensively drug resistant Mycobacterium tuberculosis strains in Peru
    Santos-Lazaro, David
    Gavilan, Ronnie G.
    Solari, Lely
    Vigo, Aiko N.
    Puyen, Zully M.
    SCIENTIFIC REPORTS, 2021, 11 (01)
  • [25] Whole-genome sequencing of Mycobacterium tuberculosis from Cambodia
    Edokimov, Konstantin
    Yamada, Yoshiyuki
    Dary, Chhavarath
    Miow, Qing Hao
    Hsu, Li-Yang
    Ong, Rick Twee-Hee
    Saphonn, Vonthanak
    SCIENTIFIC REPORTS, 2022, 12 (01)
  • [26] Whole-genome sequencing as a tool for studying the microevolution of drug-resistant serial Mycobacterium tuberculosis isolates
    do Carmo Guimaraes Diniz, Jaciara de Lourdes
    von Groll, Andrea
    Unis, Gisela
    Dalla-Costa, Elis Regina
    Rosa Rossetti, Maria Lucia
    Vianna, Julia Silveira
    Ramos, Daniela Fernandes
    Reis, Ana Julia
    Bartolomeu Halicki, Priscila Cristina
    Rheingantz Scaini, Joao Luis
    de Ibrahim das Neves, Yasmin Castillos
    Phelan, Jody
    Gomes, Ana Rita
    Campino, Susana
    Machado, Karina dos Santos
    Werhli, Adriano Velasque
    Pain, Arnab
    Clark, Taane Gregory
    Perdigao, Joao
    Viveiros, Miguel
    Portugal, Isabel
    Silva, Pedro Eduardo Almeida
    TUBERCULOSIS, 2021, 131
  • [27] Whole Genome Sequencing Based Characterization of Extensively Drug-Resistant Mycobacterium tuberculosis Isolates from Pakistan
    Ali, Asho
    Hasan, Zahra
    McNerney, Ruth
    Mallard, Kim
    Hill-Cawthorne, Grant
    Coll, Francesc
    Nair, Mridul
    Pain, Arnab
    Clark, Taane G.
    Hasan, Rumina
    PLOS ONE, 2015, 10 (02):
  • [28] Whole-genome sequencing and Mycobacterium tuberculosis: Challenges in sample preparation and sequencing data analysis
    Dohal, Matus
    Porvaznik, Igor
    Prso, Kristian
    Rasmussen, Erik Michael
    Solovic, Ivan
    Mokry, Juraj
    TUBERCULOSIS, 2020, 123
  • [29] Whole genome sequencing of clinical strains of Mycobacterium tuberculosis from Mumbai, India: A potential tool for determining drug-resistance and strain lineage
    Chatterjee, Anirvan
    Nilgiriwala, Kayzad
    Saranath, Dhananjaya
    Rodrigues, Camilla
    Mistry, Nerges
    TUBERCULOSIS, 2017, 107 : 63 - 72
  • [30] Comprehensive Whole-Genome Sequencing and Reporting of Drug Resistance Profiles on Clinical Cases of Mycobacterium tuberculosis in New York State
    Shea, Joseph
    Halse, Tanya A.
    Lapierre, Pascal
    Shudt, Matthew
    Kohlerschmidt, Donna
    Van Roey, Patrick
    Limberger, Ronald
    Taylor, Jill
    Escuyer, Vincent
    Musser, Kimberlee A.
    JOURNAL OF CLINICAL MICROBIOLOGY, 2017, 55 (06) : 1871 - 1882
12345