Requirement of CRMP2 Phosphorylation in Neuronal Migration of Developing Mouse Cerebral Cortex and Hippocampus and Redundant Roles of CRMP1 and CRMP4

被引:3
|
作者
Yamazaki, Yuki [1 ]
Moizumi, Maho [1 ]
Nagai, Jun [1 ,2 ]
Hatashita, Yoshiki [3 ]
Cai, Tianhong [1 ]
Kolattukudy, Papachan [4 ]
Inoue, Takafumi [3 ]
Goshima, Yoshio [5 ]
Ohshima, Toshio [1 ]
机构
[1] Waseda Univ, Dept Life Sci & Med Biosci, Lab Mol Brain Sci, Shinjuku Ku, 2-2 Wakamatsu Cho, Tokyo 1628480, Japan
[2] Japan Soc Promot Sci, 5-3-1 Kojimachi, Tokyo 1020083, Japan
[3] Waseda Univ, Dept Life Sci & Med Biosci, Lab Neurophysiol, Shinjuku Ku, Tokyo 1628480, Japan
[4] Univ Cent Florida, Burnett Sch Biomed Sci, 4000 Cent Florida Blvd, Orlando, FL 32816 USA
[5] Yokohama City Univ, Grad Sch Med, Dept Mol Pharmacol & Neurobiol, Yokohama, Kanagawa 2360004, Japan
基金
日本科学技术振兴机构;
关键词
phosphorylation; neuronal migration; migration; hippocampus; genetically modified mice; cerebral cortex; MICROTUBULE-ASSOCIATED PROTEIN; PYRAMIDAL NEURONS; BRAIN; DOUBLECORTIN; FAMILY; CDK5; MICE; IDENTIFICATION; DISORDERS; INTERACTS;
D O I
10.1093/cercor/bhab228
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The mammalian cerebral cortex is characterized by a 6-layer structure, and proper neuronal migration is critical for its formation. Cyclin-dependent kinase 5 (Cdk5) has been shown to be a critical kinase for neuronal migration. Several Cdk5 substrates have been suggested to be involved in ordered neuronal migration. However, in vivo loss-of-function studies on the function of Cdk5 phosphorylation substrates in neuronal migration in the developing cerebral cortex have not been reported. In this study, we demonstrated that Cdk5-mediated phosphorylation of collapsing mediator protein (CRMP) 2 is critical for neuronal migration in the developing cerebral cortex with redundant functions of CRMP1 and CRMP4. The cerebral cortices of triple-mutant CRMP1 knock-out (KO); CRMP2 knock-in (KI)/KI; and CRMP4 KO mice showed disturbed positioning of layers II-V neurons in the cerebral cortex. Further experiments using bromodeoxyuridine birthdate-labeling and in utero electroporation implicated radial migration defects in cortical neurons. Ectopic neurons were detected around the CA1 region and dentate gyrus in CRMP1 KO; CRMP2 KI/KI; and CRMP4 KO mice. These results suggest the importance of CRMP2 phosphorylation by Cdk5 and redundancy of CRMP1 and CRMP4 in proper neuronal migration in the developing cerebral cortex and hippocampus.
引用
收藏
页码:520 / 527
页数:8
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