Prediction of peptide retention volumes in gradient reversed phase HPLC

被引:2
作者
Azarova, I. N. [1 ]
Kuchkina, A. Yu. [3 ]
Baram, G. I. [1 ]
Goldberg, E. L. [2 ]
机构
[1] EcoNova Inst Chromatog, Novosibirsk 630090, Russia
[2] Novosibirsk State Univ, Novosibirsk 630090, Russia
[3] Russian Acad Sci, Siberian Branch, Inst Limnol, Irtutsk, Russia
关键词
HPLC; peptides; prediction of retention parameters;
D O I
10.1134/S1068162008020039
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A method of computation of retention volumes of linear peptides of known composition that contain no more than 25 amino acids in gradient reversed phase HPLC was developed. Tha method is suitable for various acetonitrile gradient profiles. The calculations were carried out on the basis of a statistical model, the parameters of which were experimental dependences of the retention of individual amino acids on acetonitrile concentration. The method developed was used to predict the chromatographic behavior of 34 peptides in four different acetonitrile gradients. The correlation coefficients between the predicted and experimental retention volumes were more than 0.9, and the average relative error of prediction was less than 15%.
引用
收藏
页码:156 / 161
页数:6
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