Single-cell analysis of early chick hypothalamic development reveals that hypothalamic cells are induced from prethalamic-like progenitors

被引：21

作者：

Kim, Dong Won ^{[1
]}

Place, Elsie ^{[7
,8
,9
]}

Chinnaiya, Kavitha ^{[7
,8
,9
]}

Manning, Elizabeth ^{[7
,8
,9
]}

Sun, Changyu ^{[1
]}

Dai, Weina ^{[1
]}

Ohyama, Kyoji ^{[7
,11
]}

Burbridge, Sarah ^{[7
,8
,9
]}

Placzek, Marysia ^{[7
,8
,9
]}

Blackshaw, Seth ^{[1
,2
,3
,4
,5
,6
]}

Groves, Ian ^{[10
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Solomon H Snyder Dept Neurosci, Baltimore, MD 21218 USA

[2] Johns Hopkins Univ, Sch Med, Dept Psychiat & Behav Sci, Baltimore, MD 21205 USA

[3] Johns Hopkins Univ, Sch Med, Dept Ophthalmol, Baltimore, MD 21205 USA

[4] Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21205 USA

[5] Johns Hopkins Univ, Sch Med, Inst Cell Engn, Baltimore, MD 21218 USA

[6] Johns Hopkins Univ, Sch Med, Kavli Neurosci Discovery Inst, Baltimore, MD 21218 USA

[7] Univ Sheffield, Sch Biosci, Sheffield, S Yorkshire, England

[8] Univ Sheffield, Bateson Ctr, Sheffield, S Yorkshire, England

[9] Univ Sheffield, Neurosci Inst, Sheffield, S Yorkshire, England

[10] Univ Sheffield, Sch Math & Stat, Sheffield, S Yorkshire, England

[11] Tokyo Med Univ, Dept Histol & Neuroanat, Tokyo, Japan

来源：

CELL REPORTS | 2022年 / 38卷 / 03期

基金：

英国惠康基金;

关键词：

VENTRAL MIDLINE CELLS; SONIC-HEDGEHOG; SPECIFICATION; INDUCTION; GENE; DIFFERENTIATION; EXPRESSION; NEURONS; FATE; SHH;

D O I：

10.1016/j.celrep.2021.110251

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The hypothalamus regulates many innate behaviors, but its development remains poorly understood. Here, we used single-cell RNA sequencing (RNA-seq) and hybridization chain reaction (HCR) to profile multiple stages of early hypothalamic development in the chick. Hypothalamic neuroepithelial cells are initially induced from prethalamic-like cells. Two distinct hypothalamic progenitor populations then emerge and give rise to tuberal and mammillary/paraventricular hypothalamic cells. At later stages, the regional organization of the chick and mouse hypothalamus is highly similar. We identify selective markers for major subdivisions of the developing chick hypothalamus and many previously uncharacterized candidate regulators of hypothalamic induction, regionalization, and neurogenesis. As proof of concept for the power of the data set, we demonstrate that prethalamus-derived follistatin inhibits hypothalamic induction. This study clarifies the organization of the nascent hypothalamus and identifies molecular mechanisms that may control its induction and subsequent development.

引用

页数：24