Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue

被引：213

作者：

Wang, Kai ^{[1
]}

Sun, Wenzhi ^{[1
]}

Richie, Christopher T. ^{[2
]}

Harvey, Brandon K. ^{[2
]}

Betzig, Eric ^{[1
]}

Ji, Na ^{[1
]}

机构：

[1] Howard Hughes Med Inst, Ashburn, VA 20147 USA

[2] NIDA, Intramural Res Program, Baltimore, MD 21224 USA

来源：

NATURE COMMUNICATIONS | 2015年 / 6卷

关键词：

ADAPTIVE OPTICS; FLUORESCENT PROTEINS; MICROSCOPY; BRIGHT;

D O I：

10.1038/ncomms8276

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Adaptive optics by direct imaging of the wavefront distortions of a laser-induced guide star has long been used in astronomy, and more recently in microscopy to compensate for aberrations in transparent specimens. Here we extend this approach to tissues that strongly scatter visible light by exploiting the reduced scattering of near-infrared guide stars. The method enables in vivo two-photon morphological and functional imaging down to 700 mu m inside the mouse brain.

引用

页数：6

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