Polymorphic microsatellite loci for the Balearic Island Lizard Podarcis lilfordi (Squamata: Lacertidae)

被引:7
作者
Bloor, P. [1 ,2 ]
Rodriguez, V. [3 ]
Terrasa, B. [3 ]
Brown, R. P. [4 ]
Perez-Mellado, V. [5 ]
Castro, J. A. [3 ]
Picornell, A. [3 ]
Ramon, M. M. [3 ]
机构
[1] Univ Nacl Colombia, Inst Genet, Grp Biodiversidad & Recursos Genet, Bogota, Colombia
[2] CSIC, Museo Nacl Ciencias Nat, Dept Biodiversidad & Biol Evolut, E-28006 Madrid, Spain
[3] Univ Illes Balears, Dept Biol, Genet Lab, Palma de Mallorca, Spain
[4] Liverpool John Moores Univ, Sch Nat Sci & Psychol, Liverpool L3 3AF, Merseyside, England
[5] Univ Salamanca, Dept Biol Anim, E-37008 Salamanca, Spain
关键词
Lizard; Lacertidae; Podarcis lilfordi; Microsatellite; Tetranucleotide; Balearic islands;
D O I
10.1007/s12686-010-9354-7
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
Fifteen microsatellite loci are described for the lizard Podarcis lilfordi from the Balearic Islands. Loci were isolated from partial genomic libraries that had been enriched for AAAG and ACAG repeat sequence. All loci were highly polymorphic (six to 19 alleles) in a sample of 26 individuals from the island of Dragonera. Observed and expected heterozygosities ranged from 0.46 to 0.92 and 0.78 to 0.95, respectively. These loci will be used to study the evolutionary history of different forms, the occurrence of cross-islet introductions, and the validity of subspecific designations in this species.
引用
收藏
页码:323 / 325
页数:3
相关论文
共 12 条
[1]   Highly polymorphic tetranucleotide microsatellite loci for the eastern Canary Island lizard, Gallotia atlantica [J].
Bloor, P. ;
De laguna, I. H. -B. ;
Kemp, S. J. .
MOLECULAR ECOLOGY NOTES, 2006, 6 (03) :737-739
[2]   Three islands, three worlds: Paleogeography and evolution of the vertebrate fauna from the Balearic Islands [J].
Bover, Pere ;
Quintana, Josep ;
Alcover, Josep Antoni .
QUATERNARY INTERNATIONAL, 2008, 182 :135-144
[3]   Bayesian estimation of post-Messinian divergence times in Balearic Island lizards [J].
Brown, R. P. ;
Terrasa, B. ;
Perez-Mellado, V. ;
Castro, J. A. ;
Hoskisson, P. A. ;
Picornell, A. ;
Ramon, M. M. .
MOLECULAR PHYLOGENETICS AND EVOLUTION, 2008, 48 (01) :350-358
[4]   Modulation of non-templated nucleotide addition by taq DNA polymerase: Primer modifications that facilitate genotyping [J].
Brownstein, MJ ;
Carpten, JD ;
Smith, JR .
BIOTECHNIQUES, 1996, 20 (06) :1004-+
[5]   Population density in Podarcis lilfordi (Squamata, Lacertidae), a lizard species endemic to small islets in the Balearic Islands (Spain) [J].
Perez-Mellado, Valentin ;
Angel Hernandez-Estevez, Jose ;
Garcia-Diez, Teresa ;
Terrassa, Barbara ;
Misericordia Ramon, Maria ;
Castro, Jose ;
Picornell, Antonia ;
Martin-Vallejo, Javier ;
Brown, Richard .
AMPHIBIA-REPTILIA, 2008, 29 (01) :49-60
[6]   MICROSATELLITES AND KINSHIP [J].
QUELLER, DC ;
STRASSMANN, JE ;
HUGHES, CR .
TRENDS IN ECOLOGY & EVOLUTION, 1993, 8 (08) :285-&
[7]   GENEPOP (VERSION-1.2) - POPULATION-GENETICS SOFTWARE FOR EXACT TESTS AND ECUMENICISM [J].
RAYMOND, M ;
ROUSSET, F .
JOURNAL OF HEREDITY, 1995, 86 (03) :248-249
[8]   Hybridization capture of microsatellites directly from genomic DNA [J].
Refseth, UH ;
Fangan, BM ;
Jakobsen, KS .
ELECTROPHORESIS, 1997, 18 (09) :1519-1523
[9]   ANALYZING TABLES OF STATISTICAL TESTS [J].
RICE, WR .
EVOLUTION, 1989, 43 (01) :223-225
[10]  
Rozen S, 2000, Methods Mol Biol, V132, P365