Effects of N-acetyl cysteine to improve acute lung injury in rats

被引:3
作者
Mao, L. Q. [1 ]
Li, X. B. [1 ]
机构
[1] First Peoples Hosp Taizhou, Dept Resp Med, Taizhou, Peoples R China
来源
BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY | 2017年 / 118卷 / 09期
关键词
NAC; TGF-beta; 1; TLR-4/NF-kappa B signaling pathway; lung injury; ACETYLCYSTEINE PLUS DEFEROXAMINE; OBSTRUCTIVE PULMONARY-DISEASE; HYPOXIA-INDUCED PROLIFERATION; SMOOTH-MUSCLE-CELLS; TRANSFORMING GROWTH-FACTOR-BETA-1; OXIDATIVE STRESS; APOPTOSIS; DAMAGE;
D O I
10.4149/BLL_2017_106
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
AIM: To study the mechanism of NAC to improve LPS induced acutelung lung injury. METHODS: The 40 rats were divided into 4 groups included NC group, Model group, NAC group and DXM group. The rats of Model, NAC and DXM groups were injected LPS, NAC group and DXM group were resepectively injected NAC (200 mg/kg) or DXM (70 mg/kg). Collecting the bronchoalveolar lavage fluid (BALF) from lung, and measuring the TGF-beta 1 concentration of BALF and lung tissue in 4 groups; After executing the rats, taken the lung tissue to observant lung pathological morphology and evaluated TGF-beta 1 expression of difference groups. Measuring the TLR-4 and NF-kappa b in 4 groups by WB assay. RESULTS: Compared with Model group, The NAC and DXM groups were improved in H& E staining, the TGF-beta 1 concentration of NAC and DXM groups were signifi cantly reduced in BALF and lung tissue (p < 0.05, respectively). TLR-4 and NF-kappa b proteins of NAC and DXM groups were lower than that of Model group in IHC and WB assays (p < 0.05, respectively). CONCLUSION: NAC had effects to protect LPS induced lung injury via TLR-4/NF-kappa b signaling pathway (Fig. 5, Ref. 19). Text in PDF www. elis. sk.
引用
收藏
页码:552 / 556
页数:5
相关论文
共 19 条
  • [1] The effect of N-acetylcysteine on pulmonary lipid peroxidation and tissue damage
    Akca, T
    Canbaz, H
    Tataroglu, C
    Caglikulekci, M
    Tamer, L
    Colak, T
    Kanik, A
    Bilgin, O
    Aydin, S
    [J]. JOURNAL OF SURGICAL RESEARCH, 2005, 129 (01) : 38 - 45
  • [2] Mechanisms of N-acetylcysteine in the prevention of DNA damage and cancer, with special reference to smoking-related end-points
    De Flora, S
    Izzotti, A
    D'Agostini, F
    Balansky, RM
    [J]. CARCINOGENESIS, 2001, 22 (07) : 999 - 1013
  • [3] The Role of Endoplasmic Reticulum Stress in Emphysema Results from Cigarette Smoke Exposure
    Gan, Guixiang
    Hu, Ruicheng
    Dai, Aiguo
    Tan, Shuangxiang
    Ouyang, Qing
    Fu, Daiyan
    Jiang, Dixuan
    [J]. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2011, 28 (04) : 725 - 732
  • [4] Ventilation-induced chemokine and cytokine release is associated with activation of nuclear Factor-κB and is blocked by steroids
    Held, HD
    Boettcher, S
    Hamann, L
    Uhlig, S
    [J]. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2001, 163 (03) : 711 - 716
  • [5] NOX4 mediates hypoxia-induced proliferation of human pulmonary artery smooth muscle cells: the role of autocrine production of transforming growth factor-β1 and insulin-like growth factor binding protein-3
    Ismail, Saleh
    Sturrock, Anne
    Wu, Ping
    Cahill, Barbara
    Norman, Kimberly
    Huecksteadt, Thomas
    Sanders, Karl
    Kennedy, Thomas
    Hoidal, John
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2009, 296 (03) : L489 - L499
  • [6] Valsartan attenuated oxidative stress, decreased MCP-1 and TGF-β1 expression in glomerular mesangial and epithelial cells induced by high-glucose levels
    Jiao, Bo
    Wang, Yunshan
    Cheng, Yanna
    Gao, Jianjun
    Zhang, Qingzhu
    [J]. BIOSCIENCE TRENDS, 2011, 5 (04) : 173 - 181
  • [7] Kao SJ, 2008, CLIN EXP PHARM PHYSL, V33, P33
  • [8] Transforming growth factor-beta1 upregulation triggers pulmonary artery smooth muscle cell proliferation and apoptosis imbalance in rats with hypoxic pulmonary hypertension via the PTEN/AKT pathways
    Liu, Yun
    Cao, Yonggang
    Sun, Shuyang
    Zhu, Jinquan
    Gao, Shan
    Pang, Jie
    Zhu, Daling
    Sun, Zengxian
    [J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2016, 77 : 141 - 154
  • [9] The key role of transforming growth factor-beta receptor I and 15-lipoxygenase in hypoxia-induced proliferation of pulmonary artery smooth muscle cells
    Liu, Yun
    Ma, Cui
    Zhang, Qianlong
    Yu, Lei
    Ma, Jun
    Zhang, Lei
    Hao, Xuewei
    Cao, Fangyuan
    Wang, Lin
    Zhu, Daling
    [J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2012, 44 (07) : 1184 - 1202
  • [10] Transforming growth factor-β1 causes pulmonary microvascular endothelial cell apoptosis via ALK5
    Lu, Qing
    Patel, Bhuvic
    Harrington, Elizabeth O.
    Rounds, Sharon
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2009, 296 (05) : L825 - L838