Universal primers for amplifying the complete coding sequence of cytoplasmic heat shock protein 90 (HSP90) in Lepidoptera

被引：4

作者：

Xu, Peng Jun ^{[1
,4
]}

Li, Tong ^{[1
,4
]}

Xiao, Jin Hua ^{[1
]}

Murphy, Robert W. ^{[3
,5
]}

Huang, Da Wei ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Inst Zool, Key Lab Zool Systemat & Evolut, Beijing 100101, Peoples R China

[2] Hebei Univ, Coll Life Sci, Baoding 071002, Peoples R China

[3] Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Peoples R China

[4] Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China

[5] Royal Ontario Museum, Dept Nat Hist, Toronto, ON M5S 2C6, Canada

来源：

EUROPEAN JOURNAL OF ENTOMOLOGY | 2011年 / 108卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Universal primer; Lepidoptera; coding sequence; untranslated region; HSP90; RACE; CONSTITUTIVE EXPRESSION; NUCLEOTIDE-SEQUENCE; GENE; TRANSCRIPTION; INSECTICIDES; ORFS;

D O I：

10.14411/eje.2011.020

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Using sequence alignment, a conserved domain in the 3' untranslated region (UTR) of the cytoplasmic heat shock protein 90 (HSP90) of Lepidoptera was found. This region is highly variable in other insect groups. Furthermore, universal primers were designed to amplify the complete coding sequence (CDS) of HSP90 from total genomic DNA in Lepidoptera, avoiding the commonly used reverse transcription-polymerase chain reaction (RT-PCR) and 3', 5'-rapid amplification of cDNA ends (RACE) methods based on cDNA. These primers amplified a fragment of about 2.25 kb in the 11 species tested, which represent seven different families of Lepidoptera, including moths and butterflies. The results suggest that the conserved domain of 3' UTR is universal in Lepidoptera and these primers successfully amplify the complete CDS of cytoplasmic HSP90 from genomic DNA.

引用

页码：164 / 168

页数：5

共 26 条

[1] Precise limitation of concerted evolution to ORFs in mosquito Hsp82 genes [J].

Benedict, MQ ;

Levine, BJ ;

Ke, ZX ;

Cockburn, AF ;

Seawright, JA .

INSECT MOLECULAR BIOLOGY, 1996, 5 (01) :73-79

[2]

BLACKMAN RK, 1986, J MOL BIOL, V188, P499, DOI 10.1016/S0022-2836(86)80001-8

[3] Phylogeny of phagotrophic euglenids (Euglenozoa) as inferred from hsp90 gene sequences [J].

Breglia, Susana A. ;

Slamovits, Claudio H. ;

Leander, Brian S. .

JOURNAL OF EUKARYOTIC MICROBIOLOGY, 2007, 54 (01) :86-92

[4] LONG PCR [J].

CHENG, S ;

CHANG, SY ;

GRAVITT, P ;

RESPESS, R .

NATURE, 1994, 369 (6482) :684-685

[5] Effects of neurotoxic insecticides on heat-shock proteins and cytokine transcription in Chinook salmon (Oncorhynchus tshawytscha) [J].

Eder, Kai J. ;

Leutenegger, Christian M. ;

Koehler, Heinz-R. ;

Werner, Ingeborg .

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2009, 72 (01) :182-190

[6]

Feng HZ, 2010, J INSECT SCI, V10, DOI 10.1673/031.010.11201

[7] 90-Kilodalton Heat Shock Protein, Hsp90, as a Target for Genotyping Cryptosporidium spp. Known To Infect Humans [J].

Feng, Yaoyu ;

Dearen, Theresa ;

Cama, Vitaliano ;

Xiao, Lihua .

EUKARYOTIC CELL, 2009, 8 (04) :478-482

[8] Phylogenetic analyses of the dinoflagellate Noctiluca scintillans based on β-tubulin and Hsp90 genes [J].

Fukuda, Yasuhiro ;

Endoh, Hiroshi .

EUROPEAN JOURNAL OF PROTISTOLOGY, 2008, 44 (01) :27-33

[9] Region-specific regulation of glucocorticoid receptor/HSP90 expression and interaction in brain [J].

Furay, A. R. ;

Murphy, E. K. ;

Mattson, M. P. ;

Guo, Z. ;

Herman, J. P. .

JOURNAL OF NEUROCHEMISTRY, 2006, 98 (04) :1176-1184

[10] CONSTITUTIVE EXPRESSION OF HEAT-SHOCK PROTEIN-90 (HSP90) IN NEURONS OF THE RAT-BRAIN [J].

GASS, P ;

SCHRODER, H ;

PRIOR, P ;

KIESSLING, M .

NEUROSCIENCE LETTERS, 1994, 182 (02) :188-192

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