Universal primers for amplifying the complete coding sequence of cytoplasmic heat shock protein 90 (HSP90) in Lepidoptera

被引：4

作者：

Xu, Peng Jun ^{[1
,4
]}

Li, Tong ^{[1
,4
]}

Xiao, Jin Hua ^{[1
]}

Murphy, Robert W. ^{[3
,5
]}

Huang, Da Wei ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Inst Zool, Key Lab Zool Systemat & Evolut, Beijing 100101, Peoples R China

[2] Hebei Univ, Coll Life Sci, Baoding 071002, Peoples R China

[3] Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Peoples R China

[4] Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China

[5] Royal Ontario Museum, Dept Nat Hist, Toronto, ON M5S 2C6, Canada

来源：

EUROPEAN JOURNAL OF ENTOMOLOGY | 2011年 / 108卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Universal primer; Lepidoptera; coding sequence; untranslated region; HSP90; RACE; CONSTITUTIVE EXPRESSION; NUCLEOTIDE-SEQUENCE; GENE; TRANSCRIPTION; INSECTICIDES; ORFS;

D O I：

10.14411/eje.2011.020

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Using sequence alignment, a conserved domain in the 3' untranslated region (UTR) of the cytoplasmic heat shock protein 90 (HSP90) of Lepidoptera was found. This region is highly variable in other insect groups. Furthermore, universal primers were designed to amplify the complete coding sequence (CDS) of HSP90 from total genomic DNA in Lepidoptera, avoiding the commonly used reverse transcription-polymerase chain reaction (RT-PCR) and 3', 5'-rapid amplification of cDNA ends (RACE) methods based on cDNA. These primers amplified a fragment of about 2.25 kb in the 11 species tested, which represent seven different families of Lepidoptera, including moths and butterflies. The results suggest that the conserved domain of 3' UTR is universal in Lepidoptera and these primers successfully amplify the complete CDS of cytoplasmic HSP90 from genomic DNA.

引用

页码：164 / 168

页数：5

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