Peeking into a secret world of pore-forming toxins: membrane binding processes studied by surface plasmon resonance

被引：16

作者：

Anderluh, G

Macek, P

Lakey, JH

机构：

[1] Univ Ljubljana, Biotech Fac, Dept Biol, Ljubljana 1000, Slovenia

[2] Univ Newcastle Upon Tyne, Sch Cell & Mol Biosci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England

来源：

TOXICON | 2003年 / 42卷 / 03期

关键词：

pore-forming toxin; membrane binding; surface plasmon resonance; equinatoxin;

D O I：

10.1016/S0041-0101(03)00197-1

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Pore-formation in cell membranes is used by many toxins to kill cells. It is usually a process involving multiple steps that are difficult to analyse at the molecular level. The use of surface plasmon resonance (SPR) has only recently been introduced into the study of pore-forming toxins (PFF). It can give useful data mostly on the first steps of the pore-forming process; the binding to the lipid membranes. In particular, it can make unique contributions to our knowledge of ligand specificity and the kinetics of binding. This mini-review summarizes some recent SPR studies of PFT. (C) 2003 Elsevier Ltd. All rights reserved.

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收藏

页码：225 / 228

页数：4

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