Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of zearalenone and deoxynivalenol

被引：138
作者：
Kolosova, Anna Yu
De Saeger, Sarah
Sibanda, Liberty
Verheijen, Ron
Van Peteghem, Carlos
机构：
[1] Univ Ghent, Fac Pharmaceut Sci, Lab Food Anal, B-9000 Ghent, Belgium
[2] TOXI TEST NV, B-9990 Maldegem, Belgium
[3] Euro Diagnost BV, NL-6827BN Arnhem, Netherlands
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2007年 / 389卷 / 7-8期
关键词：
lateral-flow immunoassay; colloidal gold; deoxynivalenol; zearalenone; multianalyte;
D O I：
10.1007/s00216-007-1642-z
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A multianalyte lateral-flow technique using colloidal gold-labeled monoclonal antibodies was developed for the rapid simultaneous detection of deoxynivalenol (DON) and zearalenone (ZEA). The results of this qualitative one-step test were interpreted visually. A very simple and fast sample preparation was used, and the assay procedure could be accomplished within 10 min. When applied to spiked wheat samples, the technique gave accurate and reproducible results. Cut-off levels of 1500 and 100 mu g kg(-1) for DON and ZEA, respectively, were observed. The described multianalyte format can be used as a reliable, rapid and cost-effective on-site screening technique for the simultaneous determination of mycotoxins in grain samples.
引用
收藏
页码：2103 / 2107
页数：5
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