Molecular diagnosis of bovine B-cell lymphoma using polymerase chain reaction for immunoglobulin heavy chain gene

被引：14

作者：

Maezawa, Masaki ^{[1
,2
]}

Watanabe, Ken-ichi ^{[2
]}

Horiuchi, Noriyuki ^{[1
,2
]}

Matsumoto, Kotaro ^{[1
,2
]}

Kobayashi, Yoshiyasu ^{[1
,2
]}

Inokuma, Hisashi ^{[1
,2
]}

机构：

[1] Gifu Univ, United Grad Sch Vet Sci, Gifu 5011193, Japan

[2] Obihiro Univ Agr & Vet Med, Dept Vet Med, Obihiro, Hokkaido 0808555, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2020年 / 82卷 / 01期

关键词：

bovine B-cell lymphoma; clonality; polymerase chain reaction; PCR;

D O I：

10.1292/jvms.19-0418

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We performed a clonality analysis using polymerase chain reaction (PCR) for immunoglobulin heavy chain (IgH) gene rearrangement, specifically with regard to its utility as a method to diagnose bovine B-cell lymphoma. PCR for IgH gene rearrangement indicated monoclonal proliferation of B-cells in 24 of 35 cattle with B-cell lymphoma. In contrast, PCR for IgH gene rearrangement in lymph nodes and tumor tissues from 65 cattle diagnosed with tumors other than B-cell lymphoma and non-tumors revealed polyclonal population of B-cells. Sensitivity, specificity, positive predictive value, and negative predictive value for PCR for IgH gene rearrangement for bovine B-cell lymphoma were 68.6%, 100%, 100%, and 85.5%, respectively. Clonality analysis using PCR for IgH gene rearrangement may be useful for adjunctive diagnosis of bovine B-cell lymphoma.

引用

页码：61 / 63

页数：3

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