Graft-Transmitted siRNA Signal from the Root Induces Visual Manifestation of Endogenous Post-Transcriptional Gene Silencing in the Scion

被引：36

作者：

Kasai, Atsushi ^{[1
]}

Bai, Songling ^{[1
]}

Li, Tianzhong ^{[2
]}

Harada, Takeo ^{[1
]}

机构：

[1] Hirosaki Univ, Fac Agr & Life Sci, Hirosaki, Aomori, Japan

[2] China Agr Univ, Lab Fruit Cell & Mol Breeding, Beijing 100094, Peoples R China

来源：

PLOS ONE | 2011年 / 6卷 / 02期

关键词：

RNA; MOVEMENT; PHLOEM; PLANTS; EXPRESSION; SPREAD;

D O I：

10.1371/journal.pone.0016895

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In plants, post-transcriptional gene silencing (PTGS) spreads systemically, being transmitted from the silenced stock to the scion expressing the corresponding transgene. It has been reported that a graft-transmitted siRNA signal can also induce PTGS of an endogenous gene, but this was done by top-grafting using silenced stock. In the present study involving grafting of Nicotiana benthamiana, we found that PTGS of an endogenous gene, glutamate-1-semialdehyde aminotransferase (GSA), which acts as a visible marker of RNAi via inhibition of chlorophyll synthesis, was manifested along the veins of newly developed leaves in the wild-type scion by the siRNA signal synthesized only in companion cells of the rootstock.

引用

页数：6

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