NMR Observation of Intermolecular Hydrogen Bonds between Protein Tyrosine Side-Chain OH and DNA Phosphate Groups

被引：16

作者：

Yu, Binhan ^{[1
]}

Pletka, Channing C. ^{[1
]}

Iwahara, Junji ^{[1
]}

机构：

[1] Univ Texas Med Branch, Sealy Ctr Struct Biol & Mol Biophys, Dept Biochem & Mol Biol, Galveston, TX 77555 USA

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2020年 / 124卷 / 06期

基金：

美国国家卫生研究院;

关键词：

SCALAR COUPLINGS; ELECTROSTATIC INTERACTIONS; ANTENNAPEDIA HOMEODOMAIN; HYDROXYL PROTONS; CLEANEX-PM; SPECTROSCOPY; DYNAMICS; COMPLEXES; RNA; CONFORMATION;

D O I：

10.1021/acs.jpcb.9b10987

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Hydrogen bonds between protein side-chain hydroxyl (OH) and phosphate groups are one of the most common types of intermolecular hydrogen bonds in protein-DNA/RNA complexes. Using NMR spectroscopy, we identified and characterized the hydrogen bonds between tyrosine side-chain OH and DNA phosphate groups in a protein-DNA complex. These OH groups exhibited relatively slow hydrogen-exchange rates and sizable scalar couplings between hydroxyl H-1 and DNA phosphate P-31 nuclei across the hydrogen bonds. Information about intermolecular hydrogen bonds facilitates investigations of the DNA/RNA recognition by the protein.

引用

页码：1065 / 1070

页数：6

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