Occurrence of conjugative IncF-type plasmids harboring the blaCTX-M-15 gene in Enterobacteriaceae isolates from newborns in Tunisia

被引：11
|
作者：
Lahlaoui, Hella [1 ]
De Luca, Filomena [2 ]
Maradel, Simona [2 ]
Ben-Haj-Khalifa, Anis [3 ]
Ben Hamouda, Hechmi [3 ]
Kheder, Mohamed [3 ]
Ben Moussa, Mohamed [1 ]
Rossillini, Gian-Maria [2 ]
Docquier, Jean-Denis [2 ]
机构：
[1] Mil Hosp Tunis, Microbiol Lab, Monfleury, Tunisia
[2] Univ Siena, Dept Med Biotechnol, I-53100 Siena, Italy
[3] Hosp Tahar Sfar, Microbiol Lab, Mahdia, Tunisia
来源：
PEDIATRIC RESEARCH | 2015年 / 77卷 / 01期
关键词：
SPECTRUM BETA-LACTAMASES; CTX-M-TYPE; COMPLETE NUCLEOTIDE-SEQUENCE; ESCHERICHIA-COLI; KLEBSIELLA-PNEUMONIAE; ADDICTION SYSTEMS; DISSEMINATION; CTX-M-15; STRAINS; PREVALENCE;
D O I：
10.1038/pr.2014.153
中图分类号：
R72 [儿科学];
学科分类号：
100202 ;
摘要：
BACKGROUND: CTX-M-15 is the dominant type of extended-spectrum beta-lactamase in clinical isolates. This enzyme constitutes the most widespread enzymes in Tunisia. In this study, we were interested to understand the causes of the evolutionary success of CTX-M-15 in a Tunisian university hospital. METHODS: A total of of 72 cefotaxime-resistant Enterobacteriaceae were isolated from newborn patients at the hospital Taher sfar Mandia in Tunisia and characterized their genetic support by means of molecular techniques. RESULTS: Isolates were clustered into various clonal groups, although most isolates belonged to sequence types ST39 (Klebsiella pneumoniae) and ST131 (Escherichia coli). F replicons (FIA, FIB, and Fit) were the most frequently detected replicon types in our collection (91.66%). CONCLUSION: This is the first report of QnrB- and CTX-M-15-encoding large IncF-type conjugative plasmids in Tunisia.
引用
收藏
页码：107 / 110
页数：4
相关论文
共 38 条
  • [1] blaCTX-M-15 carried by IncF-type plasmids is the dominant ESBL gene in Escherichia coli and Klebsiella pneumoniae at a hospital in Ghana
    Agyekum, Alex
    Fajardo-Lubian, Alicia
    Ansong, Daniel
    Partridge, Sally R.
    Agbenyega, Tsiri
    Iredell, Jonathan R.
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2016, 84 (04) : 328 - 333
  • [2] Recombination in IS26 and Tn2 in the Evolution of Multiresistance Regions Carrying blaCTX-M-15 on Conjugative IncF Plasmids from Escherichia coli
    Partridge, Sally R.
    Zong, Zhiyong
    Iredell, Jonathan R.
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2011, 55 (11) : 4971 - 4978
  • [3] Replicon typing of plasmids carrying blaCTX-M-15 among Enterobacteriaceae isolated at the environment, livestock and human interface
    Zurfluh, Katrin
    Glier, Melinda
    Haechler, Herbert
    Stephan, Roger
    SCIENCE OF THE TOTAL ENVIRONMENT, 2015, 521 : 75 - 78
  • [4] Dissemination of IncF-type plasmids in multiresistant CTX-M-15-producing Enterobacteriaceae isolates from surgical-site infections in Bangui, Central African Republic
    Rafai, Clotaire
    Frank, Thierry
    Manirakiza, Alexandre
    Gaudeuille, Alfred
    Mbecko, Jean-Robert
    Nghario, Luc
    Serdouma, Eugene
    Tekpa, Bertrand
    Garin, Benoit
    Breurec, Sebastien
    BMC MICROBIOLOGY, 2015, 15
  • [5] Genetic heterogeneity and predominance of blaCTX-M-15 in cefotaxime-resistant Enterobacteriaceae isolates colonizing hospitalized children in Tunisia
    Harbaoui, S.
    Ferjani, S.
    Abbassi, M. S.
    Saidani, M.
    Gargueh, T.
    Ferjani, M.
    Hammi, Y.
    Boutiba-Ben Boubaker, I
    LETTERS IN APPLIED MICROBIOLOGY, 2022, 75 (06) : 1460 - 1474
  • [6] RETRACTED: Diverse Plasmids Harboring blaCTX-M-15 in Klebsiella pneumoniae ST11 Isolates from Several Asian Countries (Retracted Article)
    Kim, So Yeon
    Ko, Kwan Soo
    MICROBIAL DRUG RESISTANCE, 2019, 25 (02) : 227 - 232
  • [7] IncH-Type Plasmid Harboring blaCTX-M-15, blaDHA-1, and qnrB4 Genes Recovered from Animal Isolates
    Schlueter, Andreas
    Nordmann, Patrice
    Bonnin, Remy A.
    Millemann, Yves
    Eikmeyer, Felix G.
    Wibberg, Daniel
    Puehler, Alfred
    Poirel, Laurent
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2014, 58 (07) : 3768 - 3773
  • [8] Various Inc-type plasmids and lineages of Escherichia coli and Klebsiella pneumoniae spreading blaCTX-M-15, blaCTX-M-1 and mcr-1 genes in camels in Tunisia
    Saidani, Mariem
    Messadi, Lilia
    Mefteh, Jomaa
    Chaouechi, Amira
    Soudani, Alya
    Selmi, Rachid
    Daaloul-Jedidi, Monia
    Ben Chehida, Faten
    Mamlouk, Aymen
    Jemli, Mohamed Habib
    Madec, Jean-Yves
    Haenni, Marisa
    JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 2019, 19 : 280 - 283
  • [9] Dissemination of the blaCTX-M-15 gene among Enterobacteriaceae via outer membrane vesicles
    Bielaszewska, Martina
    Daniel, Ondrej
    Karch, Helge
    Mellmann, Alexander
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2020, 75 (09) : 2442 - 2451
  • [10] Increasing secondary bacterial infections with Enterobacteriaceae harboring blaCTX-M-15 and blaCMY-6 in patients with bronchogenic carcinoma: an emerging point of concern
    Shahid, Mohammed
    Malik, Abida
    Bhargava, Rakesh
    ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE, 2011, 4 (01) : 5 - 12
1234