Analysis of Neurotransmitter Tissue Content of Drosophila melanogaster in Different Life Stages

被引：32

作者：

Denno, Madelaine E. ^{[1
]}

Privman, Eve ^{[1
,2
]}

Venton, B. Jill ^{[1
,2
,3
]}

机构：

[1] Univ Virginia, Dept Chem, Charlottesville, VA 22904 USA

[2] Univ Virginia, Grad Program Neurosci, Charlottesville, VA 22904 USA

[3] Univ Virginia, Med Scientist Training Program, Charlottesville, VA 22904 USA

来源：

ACS CHEMICAL NEUROSCIENCE | 2015年 / 6卷 / 01期

关键词：

Drosophila melanogaster; development; tissue content; capillary electrophoresis; neurotransmitters; AMPLIFIED SAMPLE STACKING; BIOGENIC-AMINES; CAPILLARY-ELECTROPHORESIS; BRAIN-DEVELOPMENT; SEX-DIFFERENCES; DOPAMINE; EXPRESSION; OCTOPAMINE; SEROTONIN; GENE;

D O I：

10.1021/cn500261e

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Drosophila melanogaster is a widely used model organism for studying neurological diseases with similar neurotransmission to mammals. While both larva and adult Drosophila have central nervous systems, not much is known about how neurotransmitter tissue content changes through development. In this study, we quantified tyramine, serotonin, octopamine, and dopamine in larval, pupal, and adult fly brains using capillary electrophoresis coupled to fast-scan cyclic voltammetry. Tyramine and octopamine content varied between life stages, with almost no octopamine being present in the pupa, while tyramine levels in the pupa were very high. Adult females had significantly higher dopamine content than males, but no other neurotransmitters were dependent on sex in the adult. Understanding the tissue content of different life stages will be beneficial for future work comparing the effects of diseases on tissue content throughout development.

引用

页码：117 / 123

页数：7

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