Expression of the gene coding for bacterial hemoglobin improves β-galactosidase production in a recombinant Pichia pastoris

被引：26
|
作者：
Wu, JM
Hsu, TA
Lee, CK [1 ]
机构：
[1] Natl Taiwan Univ Sci & Technol, Dept Chem Engn, Taipei 106, Taiwan
[2] Natl Hlth Res Inst, Div Biotechnol & Pharmaceut Res, Taipei 115, Taiwan
[3] Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu 300, Taiwan
来源：
BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 17期
关键词：
alcohol oxidase1 promoter; bacterial hemoglobin; beta-galactosidase; peroxisome; recombinant Pichia pastoris;
D O I：
10.1023/A:1025072020791
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Genes coding for Vitreoscilla hemoglobin (VHb) with peroxisome targeting signal (PTS1) tag and beta-galactosidase were co-expressed in Pichia pastoris under the alcohol oxidase1 (AOX1) promoter. The expression of VHb-PTS1 had no positive effect on cell growth but significantly enhanced the whole cell beta-galactosidase activity to 4-fold higher than that of VHb-free cell in yeast extract/peptone/methanol medium under aerobic cultivation.
引用
收藏
页码：1457 / 1462
页数：6
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