Evaluation of the RapidHIT™ 200 and RapidHIT GlobalFiler® Express kit for fully automated STR genotyping

被引：17
作者：
Date-Chong, Mavis [1 ]
Hudlow, William R. [1 ]
Buoncristiani, Martin R. [1 ]
机构：
[1] Calif Dept Justice, Jan Bashinski DNA Lab, Bur Forens Serv, Richmond, CA 94804 USA
来源：
FORENSIC SCIENCE INTERNATIONAL-GENETICS | 2016年 / 23卷
关键词：
RapidHIT (TM) 200; Rapid DNA; Modified Rapid DNA analysis; GlobalFiler (R) Express; HUMAN IDENTIFICATION SYSTEM; DEVELOPMENTAL VALIDATION; SAMPLE;
D O I：
10.1016/j.fsigen.2016.03.001
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The RapidHIT (TM) 200 Human Identification System and RapidHIT GlobalFiler (R) Express kit were evaluated and validated for use with single-source reference samples. It was of primary interest to evaluate the system for its efficacy as an expert system and to estimate a first pass success rate, as well as to identify the technical variables impacting that result. While results indicated that this instrument/kit combination can be used to accurately type single-source buccal samples, substantial variability in sensitivity and intra-color balance were observed, as were multiple artifacts, requiring extensive manual editing of the profiles. Artifacts included dye "blobs" and spectral overlap (pull-up) peaks that often originated from relatively low intensity allele peaks. Reduced intra-color balance, in combination with low sensitivity, occasionally resulted in instances of allelic dropout. Overall, 50% of the buccal samples analyzed in this study would have been successfully typed to give full GlobalFiler (R) profiles without the need for manual review and editing. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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页码：1 / 8
页数：8
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