Intra-session repeatability of anterior chamber depth across the chamber width using Pentacam Scheimpflug imaging in healthy subjects

Purpose Despite the importance of anterior chamber depth (ACD) measurements in disease and ageing, the repeatability and their threshold for change is not known. Our purpose was to determine the intra-session repeatability of Pentacam Scheimpflug photography for measuring the ACD across the chamber width in healthy subjects and thus inform expected limits of normality. Methods Pentacam Scheimpflug photography was used to obtain ACD measurements at 57 points across the central 8mm of the chamber width from one randomly selected eye of 130 healthy (normal vision and no ocular diseases, except age-normal cataracts) subjects (median age 58.0 years, interquartile range 46.3-63.0 years; 48 males, 82 females). Intra-session ACD measurements were compared. Univariate and multivariate linear regression was performed to identify categorical and continuous variables demonstrating a significant relationship with ACD and its repeatability. Results Bland-Altman analyses showed no directional or depth-dependent bias in the difference between the first and second tests (mean bias -0.003 mm, 95% limits of agreement -0.115 to +0.109 mm). Multivariate analysis found gender to be a significant factor (p < 0.0001), but not age (p = 0.69) nor ethnicity (p = 0.65), although the model fit was poor (R-2 = 0.004). There were no regional differences in repeatability measures found in males, but six locations in the superior aspect in females were found to be significantly different in their repeatability characteristics. Tolerance limits used to calculate the number of step sizes between 60-year-old age groups found 8.1-11.5 steps for females, and 7.5-9.2 steps for males. Conclusions Scheimpflug imaging using the Pentacam has excellent intra-session repeatability. Only gender appeared to affect repeatability characteristics, manifesting with a greater number of meaningful steps of change between two extremes of age range in females compared to males, which provides guidance for identifying clinically significant and measurable change between tests.