Dual-view photoacoustic microscopy for quantitative cell nuclear imaging

被引：19
|
作者：
Cai, De [1 ,2 ]
Wong, Terence T. W. [1 ,3 ]
Zhu, Liren [1 ]
Shi, Junhui [1 ]
Chen, Sung-Liang [2 ]
Wang, Lihong V. [1 ]
机构：
[1] CALTECH, Opt Imaging Lab, Andrew & Peggy Cherng Dept Med Engn, Dept Elect Engn, Pasadena, CA 91125 USA
[2] Shanghai Jiao Tong Univ, Univ Michigan Shanghai Jiao Tong Univ Joint Inst, Shanghai 200240, Peoples R China
[3] Hong Kong Univ Sci & Technol, Dept Chem & Biol Engn, Translat & Adv Bioimaging Lab, Hong Kong, Hong Kong, Peoples R China
来源：
OPTICS LETTERS | 2018年 / 43卷 / 20期
基金：
美国国家卫生研究院;
关键词：
PLANE ILLUMINATION MICROSCOPY; CANCER-CELLS; RESOLUTION; TOMOGRAPHY;
D O I：
10.1364/OL.43.004875
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging imaging modality for studying biological tissues. However, in conventional single-view OR-PAM, the lateral and axial resolutions-determined optically and acoustically, respectively-are highly anisotropic. In this Letter, we introduce dual-view OR-PAM to improve axial resolution, achieving three-dimensional (3D) resolution isotropy. We first use 0.5 mu m polystyrene beads and carbon fibers to validate the resolution isotropy improvement. Imaging of mouse brain slices further demonstrates the improved resolution isotropy, revealing the 3D structure of cell nuclei in detail, which facilitates quantitative cell nuclear analysis. (C) 2018 Optical Society of America
引用
收藏
页码：4875 / 4878
页数：4
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