In Vitro Assessment of the Genotoxic Potential of Pristine Graphene Platelets

被引:9
作者
Malkova, Andrea [1 ,2 ]
Svadlakova, Tereza [1 ,3 ,4 ]
Singh, Avni [3 ,4 ]
Kolackova, Martina [3 ,4 ]
Vankova, Radka [3 ,4 ]
Borsky, Pavel [1 ,2 ]
Holmannova, Drahomira [1 ]
Karas, Adam [1 ]
Borska, Lenka [1 ]
Fiala, Zdenek [1 ]
机构
[1] Charles Univ Prague, Fac Med Hradec Kralove, Inst Prevent Med, Hradec Kralove 50003, Czech Republic
[2] Charles Univ Prague, Fac Med Hradec Kralove, Inst Pathol Physiol, Hradec Kralove 50003, Czech Republic
[3] Charles Univ Prague, Univ Hosp Hradec Kralove, Inst Clin Immunol & Allergol, Hradec Kralove 50005, Czech Republic
[4] Charles Univ Prague, Fac Med Hradec Kralove, Hradec Kralove 50005, Czech Republic
关键词
pristine graphene platelets; THP-1; genotoxicity; cytotoxicity; oxidative stress; immunotoxicity; micronucleus test; NANOPLATELETS; OXIDE; NANOPARTICLES; CYTOTOXICITY; TOXICITY; CELLS; ASSAY; SINGLE; VIVO;
D O I
10.3390/nano11092210
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
(1) Background: Graphene is a two-dimensional atomic structure with a wide range of uses, including for biomedical applications. However, knowledge of its hazards is still limited. This work brings new cytotoxic, cytostatic, genotoxic and immunotoxic data concerning the in vitro exposure of human cell line to two types of graphene platelets (GP). It also contributes to the formation of general conclusions about the health risks of GP exposure. (2) Methods: In vitro exposure of a THP-1 cell line to three concentrations of two GP over 40 h. The cytotoxic potential was assessed by the measurement of LDH and glutathione (ROS) and by a trypan blue exclusion assay (TBEA); the cytostatic and genotoxic potential were assessed by the cytokinesis-block micronucleus (CBMN) test; and the immunotoxic potential was assessed by the measurement of IL-6, IL-10 and TNF-alpha. (3) Results: We found a significant dose-dependent increase in DNA damage (CBMN). The lowest observed genotoxic effect levels (LOGEL) were 5 mu g/mL (GP1) and 30 mu g/mL (GP2). We found no significant leaking of LDH from cells, increase in dead cells (TBEA), induction of ROS, increased levels of cytostasis, or changes in IL-6, IL-10 and TNF-alpha levels. (4) Conclusions: The genotoxicity increased during the short-term in vitro exposure of THP-1 to two GP. No increase in cytotoxicity, immunotoxicity, or cytostasis was observed.
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页数:16
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